A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate

Acetoin (3-hydroxy-2-butanone) is an important bio-based platform chemical with wide applications. In vitro enzyme catalysed synthesis exhibits great feasibility in the production of chemicals with high purity. In the present work, a synthetic pathway involving a two-step continuous reaction was con...

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Bibliographic Details
Published in:	Scientific reports Vol. 7; no. 1; pp. 4333 - 10
Main Authors:	Jia, Xiaojing, Liu, Ying, Han, Yejun
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 28-06-2017 Nature Publishing Group Nature Portfolio
Subjects:	38/22 38/23 38/77 631/45/603 631/61/338/552 82/29 82/80 82/83 Acetoin Acetoin - metabolism Acetolactate decarboxylase Acetolactate synthase Acetolactate Synthase - genetics Acetolactate Synthase - metabolism Biosynthetic Pathways Catalysis Enzyme Activation Enzyme kinetics Fermentation Gene Expression Regulation, Enzymologic Humanities and Social Sciences Hydrogen-Ion Concentration Kinetics multidisciplinary NADP pH effects Pyruvic acid Pyruvic Acid - metabolism Science Science (multidisciplinary)
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