CLEC14a-HSP70-1A interaction regulates HSP70-1A-induced angiogenesis

• Published in: Scientific reports Vol. 7; no. 1; pp. 10666 - 12
• Main Authors: Jang, Jihye, Kim, Mi Ra, Kim, Taek-Keun, Lee, Woo Ran, Kim, Jong Heon, Heo, Kyun, Lee, Sukmook
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06-09-2017; Nature Publishing Group; Nature Portfolio
• Subjects: 13/106; 13/31; 14/34; 38/1; 631/45/612/1237; 631/67/2328; 82/103; 82/29; 82/58; 82/80; 82/83; Amino acids; Angiogenesis; Endothelial cells; Extracellular signal-regulated kinase; Heat shock proteins; Hsp70 protein; Humanities and Social Sciences; Immunoprecipitation; Molecular modelling; multidisciplinary; Phosphorylation; Proteins; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-11118-y

CLEC14a (C-type lectin domain family 14 member) is a tumor endothelial cell marker protein that is known to play an important role in tumor angiogenesis, but the basic molecular mechanisms underlying this function have not yet been clearly elucidated. In this study, using various proteomic tools, we isolated a 70-kDa protein that interacts with the C-type lectin-like domain of CLEC14a (CLEC14a-CTLD) and identified it as heat shock protein 70-1A (HSP70-1A). Co-immunoprecipitation showed that HSP70-1A and CLEC14a interact on endothelial cells.
In vitro
binding analyses identified that HSP70-1A specifically associates with the region between amino acids 43 and 69 of CLEC14a-CTLD. Competitive blocking experiments indicated that this interacting region of CLEC14a-CTLD significantly inhibits HSP70-1A-induced extracellular signal-regulated kinase (ERK) phosphorylation and endothelial tube formation by directly inhibiting CLEC14a-CTLD-mediated endothelial cell-cell contacts. Our data suggest that the specific interaction of HSP70-1A with CLEC14a may play a critical role in HSP70-1A-induced angiogenesis and that the HSP70-1A-interacting region of CLEC14a-CTLD may be a useful tool for inhibiting HSP70-1A-induced angiogenesis.