Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy

Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5]oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8),...

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Bibliographic Details
Published in:	Bioorganic & medicinal chemistry letters Vol. 21; no. 18; pp. 5347 - 5352
Main Authors:	Taldone, Tony, Gomes-DaGama, Erica M., Zong, Hongliang, Sen, Siddhartha, Alpaugh, Mary L., Zatorska, Danuta, Alonso-Sabadell, Raul, Guzman, Monica L., Chiosis, Gabriela
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Ltd 15-09-2011 Elsevier
Subjects:	Antineoplastic agents Biological and medical sciences Cell Line, Tumor Chemistry Techniques, Synthetic Flow Cytometry fluorescein fluorescence Fluorescence microscopy Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Fluorescent Hsp90 inhibitor General aspects heat shock proteins HSP90 Heat-Shock Proteins - analysis Humans Medical sciences Microscopy, Fluorescence Molecular Structure Pharmacology. Drug treatments PU-H71 Purine Purines - chemistry Stereoisomerism Flow cytometry Purine PU-H71 Fluorescent Hsp90 inhibitor Fluorescence microscopy Antineoplastic agent Purine derivatives Acute myelogenous leukemia Leukemia Cytotoxicity Confocal microscopy Iodine Organic compounds Signal transduction Scanning microscope Heat shock protein Chemical synthesis Tumor cell Heat shock protein 90 Adenine derivatives Human Cell permeability Enzyme Chaperone Adenosinetriphosphatase Enzyme inhibitor In vitro Cell line Fluorescent labelling Hydrolases Confocal laser scanning microscopy
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Summary:	Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5]oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8), were shown to be suitable for fluorescence-activated flow cytometry and fluorescence microscopy. Thus these molecules serve as useful probes for studying Hsp90 in heterogeneous live cell populations.
Bibliography:	http://dx.doi.org/10.1016/j.bmcl.2011.07.026 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0960-894X 1464-3405
DOI:	10.1016/j.bmcl.2011.07.026