Effects of diode laser therapy on the acellular dermal matrix

• Published in: Cell and tissue banking Vol. 10; no. 4; pp. 327 - 332
• Main Authors: Soares, Lívia Prates, de Oliveira, Marília Gerhardt, de Almeida Reis, Sílvia Regina
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-11-2009; Springer; Springer Nature B.V
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Bone marrow, stem cells transplantation. Graft versus host reaction; Cell Biology; Clinical outcomes; Collagen - therapeutic use; Dental surgery; Fibroblasts - cytology; Graft Survival; Lasers; Lasers, Semiconductor - therapeutic use; Life Sciences; Low-Level Light Therapy; Male; Medical sciences; Rats; Rats, Wistar; Skin & tissue grafts; Skin Transplantation; Skin, Artificial; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplant Surgery; Transplantation, Heterologous; Transplants & implants; Wound Healing; Biostimulation; Xenograft; Dermal implant; Low-level laser; Implant; Tissue bank; Transplantation; Heterograft; Heterotransplantation; Tissue; Treatment; Acellular derm; Low; Implanted; Surgery; Animal; Laser; Graft; Skin; Cell
• ISSN: 1389-9333; 1573-6814
• DOI: 10.1007/s10561-009-9135-2

Acellular dermal matrix (ADM) was subcutaneously implanted into calvarian skin of male Wistar rats (
n
 = 40). Low-level laser (λ 685 nm, 4 J/cm
2
) was locally applied in experimental group (
n
 = 20) above the skin flap. Grafts were harvested at 1, 3, 7 and 14 days after surgery and underwent histological analyses. In treated animals, the extent of edema and the number of inflammatory cells were reduced (
P
 < 0.05). The amount of collagen in graft treated with low-level laser were significantly higher than those of controls (
P
 < 0.05) and were statistically more prominent on the 14th day after surgery. The mean count of fibroblasts was significantly higher in the low-laser therapy group within the 3rd day, showing a marked influx of fibroblasts into area. In conclusion, wound healing of the ADM appear to be positively affected by laser therapy.