Preparation of biologically activated lignite immobilized SRB particles and their AMD treatment characteristics

In response to the insufficient supply of carbon sources and the toxicity of heavy metal ions when using sulfate reducing bacteria (SRB) to treat acid mine wastewater (AMD), the immobilized particles are prepared with Rhodopseudomonas , SRB and lignite as the main raw materials. And based on single...

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Published in:Scientific reports Vol. 12; no. 1; p. 3964
Main Authors: Di, Junzhen, Jiang, Yangyang, Wang, Mingjia, Dong, Yanrong
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Published: London Nature Publishing Group UK 10-03-2022
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Abstract In response to the insufficient supply of carbon sources and the toxicity of heavy metal ions when using sulfate reducing bacteria (SRB) to treat acid mine wastewater (AMD), the immobilized particles are prepared with Rhodopseudomonas , SRB and lignite as the main raw materials. And based on single factor test and orthogonal test to determine the optimal ratio of biologically activated lignite fixed SRB particles. The adsorption characteristics of immobilized particles were studied under the optimal ratio, and the reaction kinetics and adsorption capacity of SRB particles immobilized on biologically activated lignite to different ions were analyzed. The results show that: lignite not only has good adsorption performance, but also can be used as the carbon source of SRB after being degraded by Rhodopseudomonas , solving the problems of low removal efficiency of SRB treatment of AMD and insufficient carbon source supply. When the dosage of lignite (particle size is 200 mesh), Rhodopseudomonas , and SRB are 3%, 10%, and 10% mesh, the prepared biologically activated lignite-immobilized SRB particles have the best effect on AMD treatment. The removal rates of SO 4 2− , Zn 2+ , and Cu 2+ were 83.21%, 99.59%, and 99.93%, respectively, the pH was increased to 7.43, the COD release was 523 mg/L, and the ORP value was − 134 mV. The reduction process of SO 4 2− by the biologically activated lignite-immobilized SRB particles conforms to the pseudo-first-order kinetics, and the adsorption of Zn 2+ is more in line with the Freundlich isotherm adsorption equation and the pseudo-second-order kinetic model. And it does not spread in a single form, both internal and external diffusion occur. SEM, FT-IR, and BET analysis of biologically activated lignite immobilized SRB particles showed that the pore structure is developed, has a large number of adsorption sites, and some activated groups participate in the reaction. The adsorption process of Zn 2+ and Cu 2+ in AMD meets the multi-layer adsorption theory.
AbstractList In response to the insufficient supply of carbon sources and the toxicity of heavy metal ions when using sulfate reducing bacteria (SRB) to treat acid mine wastewater (AMD), the immobilized particles are prepared with Rhodopseudomonas , SRB and lignite as the main raw materials. And based on single factor test and orthogonal test to determine the optimal ratio of biologically activated lignite fixed SRB particles. The adsorption characteristics of immobilized particles were studied under the optimal ratio, and the reaction kinetics and adsorption capacity of SRB particles immobilized on biologically activated lignite to different ions were analyzed. The results show that: lignite not only has good adsorption performance, but also can be used as the carbon source of SRB after being degraded by Rhodopseudomonas , solving the problems of low removal efficiency of SRB treatment of AMD and insufficient carbon source supply. When the dosage of lignite (particle size is 200 mesh), Rhodopseudomonas , and SRB are 3%, 10%, and 10% mesh, the prepared biologically activated lignite-immobilized SRB particles have the best effect on AMD treatment. The removal rates of SO 4 2− , Zn 2+ , and Cu 2+ were 83.21%, 99.59%, and 99.93%, respectively, the pH was increased to 7.43, the COD release was 523 mg/L, and the ORP value was − 134 mV. The reduction process of SO 4 2− by the biologically activated lignite-immobilized SRB particles conforms to the pseudo-first-order kinetics, and the adsorption of Zn 2+ is more in line with the Freundlich isotherm adsorption equation and the pseudo-second-order kinetic model. And it does not spread in a single form, both internal and external diffusion occur. SEM, FT-IR, and BET analysis of biologically activated lignite immobilized SRB particles showed that the pore structure is developed, has a large number of adsorption sites, and some activated groups participate in the reaction. The adsorption process of Zn 2+ and Cu 2+ in AMD meets the multi-layer adsorption theory.
In response to the insufficient supply of carbon sources and the toxicity of heavy metal ions when using sulfate reducing bacteria (SRB) to treat acid mine wastewater (AMD), the immobilized particles are prepared with Rhodopseudomonas, SRB and lignite as the main raw materials. And based on single factor test and orthogonal test to determine the optimal ratio of biologically activated lignite fixed SRB particles. The adsorption characteristics of immobilized particles were studied under the optimal ratio, and the reaction kinetics and adsorption capacity of SRB particles immobilized on biologically activated lignite to different ions were analyzed. The results show that: lignite not only has good adsorption performance, but also can be used as the carbon source of SRB after being degraded by Rhodopseudomonas, solving the problems of low removal efficiency of SRB treatment of AMD and insufficient carbon source supply. When the dosage of lignite (particle size is 200 mesh), Rhodopseudomonas, and SRB are 3%, 10%, and 10% mesh, the prepared biologically activated lignite-immobilized SRB particles have the best effect on AMD treatment. The removal rates of SO42−, Zn2+, and Cu2+ were 83.21%, 99.59%, and 99.93%, respectively, the pH was increased to 7.43, the COD release was 523 mg/L, and the ORP value was − 134 mV. The reduction process of SO42− by the biologically activated lignite-immobilized SRB particles conforms to the pseudo-first-order kinetics, and the adsorption of Zn2+ is more in line with the Freundlich isotherm adsorption equation and the pseudo-second-order kinetic model. And it does not spread in a single form, both internal and external diffusion occur. SEM, FT-IR, and BET analysis of biologically activated lignite immobilized SRB particles showed that the pore structure is developed, has a large number of adsorption sites, and some activated groups participate in the reaction. The adsorption process of Zn2+ and Cu2+ in AMD meets the multi-layer adsorption theory.
Abstract In response to the insufficient supply of carbon sources and the toxicity of heavy metal ions when using sulfate reducing bacteria (SRB) to treat acid mine wastewater (AMD), the immobilized particles are prepared with Rhodopseudomonas, SRB and lignite as the main raw materials. And based on single factor test and orthogonal test to determine the optimal ratio of biologically activated lignite fixed SRB particles. The adsorption characteristics of immobilized particles were studied under the optimal ratio, and the reaction kinetics and adsorption capacity of SRB particles immobilized on biologically activated lignite to different ions were analyzed. The results show that: lignite not only has good adsorption performance, but also can be used as the carbon source of SRB after being degraded by Rhodopseudomonas, solving the problems of low removal efficiency of SRB treatment of AMD and insufficient carbon source supply. When the dosage of lignite (particle size is 200 mesh), Rhodopseudomonas, and SRB are 3%, 10%, and 10% mesh, the prepared biologically activated lignite-immobilized SRB particles have the best effect on AMD treatment. The removal rates of SO4 2−, Zn2+, and Cu2+ were 83.21%, 99.59%, and 99.93%, respectively, the pH was increased to 7.43, the COD release was 523 mg/L, and the ORP value was − 134 mV. The reduction process of SO4 2− by the biologically activated lignite-immobilized SRB particles conforms to the pseudo-first-order kinetics, and the adsorption of Zn2+ is more in line with the Freundlich isotherm adsorption equation and the pseudo-second-order kinetic model. And it does not spread in a single form, both internal and external diffusion occur. SEM, FT-IR, and BET analysis of biologically activated lignite immobilized SRB particles showed that the pore structure is developed, has a large number of adsorption sites, and some activated groups participate in the reaction. The adsorption process of Zn2+ and Cu2+ in AMD meets the multi-layer adsorption theory.
In response to the insufficient supply of carbon sources and the toxicity of heavy metal ions when using sulfate reducing bacteria (SRB) to treat acid mine wastewater (AMD), the immobilized particles are prepared with Rhodopseudomonas, SRB and lignite as the main raw materials. And based on single factor test and orthogonal test to determine the optimal ratio of biologically activated lignite fixed SRB particles. The adsorption characteristics of immobilized particles were studied under the optimal ratio, and the reaction kinetics and adsorption capacity of SRB particles immobilized on biologically activated lignite to different ions were analyzed. The results show that: lignite not only has good adsorption performance, but also can be used as the carbon source of SRB after being degraded by Rhodopseudomonas, solving the problems of low removal efficiency of SRB treatment of AMD and insufficient carbon source supply. When the dosage of lignite (particle size is 200 mesh), Rhodopseudomonas, and SRB are 3%, 10%, and 10% mesh, the prepared biologically activated lignite-immobilized SRB particles have the best effect on AMD treatment. The removal rates of SO , Zn , and Cu were 83.21%, 99.59%, and 99.93%, respectively, the pH was increased to 7.43, the COD release was 523 mg/L, and the ORP value was - 134 mV. The reduction process of SO by the biologically activated lignite-immobilized SRB particles conforms to the pseudo-first-order kinetics, and the adsorption of Zn is more in line with the Freundlich isotherm adsorption equation and the pseudo-second-order kinetic model. And it does not spread in a single form, both internal and external diffusion occur. SEM, FT-IR, and BET analysis of biologically activated lignite immobilized SRB particles showed that the pore structure is developed, has a large number of adsorption sites, and some activated groups participate in the reaction. The adsorption process of Zn and Cu in AMD meets the multi-layer adsorption theory.
ArticleNumber 3964
Author Wang, Mingjia
Di, Junzhen
Dong, Yanrong
Jiang, Yangyang
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/35273309$$D View this record in MEDLINE/PubMed
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Snippet In response to the insufficient supply of carbon sources and the toxicity of heavy metal ions when using sulfate reducing bacteria (SRB) to treat acid mine...
Abstract In response to the insufficient supply of carbon sources and the toxicity of heavy metal ions when using sulfate reducing bacteria (SRB) to treat acid...
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SubjectTerms 631/326
639/638
704/172
Adsorption
Biological analysis
Carbon
Carbon - chemistry
Carbon sources
Coal
Copper
Heavy metals
Humanities and Social Sciences
Hydrogen-Ion Concentration
Kinetics
Lignite
Metal ions
multidisciplinary
Raw materials
Rhodopseudomonas
Science
Science (multidisciplinary)
Spectroscopy, Fourier Transform Infrared
Sulfate reduction
Sulfate-reducing bacteria
Toxicity
Waste Water - chemistry
Wastewater
Water Pollutants, Chemical - analysis
Zinc
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Title Preparation of biologically activated lignite immobilized SRB particles and their AMD treatment characteristics
URI https://link.springer.com/article/10.1038/s41598-022-08029-y
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