Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1

• Published in: Nature communications Vol. 13; no. 1; p. 1294
• Main Authors: Fan, Li, Fröhlich, Katja, Melzer, Eric, Pruitt, Rory N., Albert, Isabell, Zhang, Lisha, Joe, Anna, Hua, Chenlei, Song, Yanyue, Albert, Markus, Kim, Sang-Tae, Weigel, Detlef, Zipfel, Cyril, Chae, Eunyoung, Gust, Andrea A., Nürnberger, Thorsten
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11-03-2022; Nature Publishing Group; Nature Portfolio
• Subjects: 38/1; 38/70; 38/77; 45/43; 631/449/2169/2107; 631/449/2169/2674; 631/45/611; 82/83; Arabidopsis; Arabidopsis - metabolism; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; DNA sequencing; Epitopes; Genotype; Genotypes; Genotyping; Humanities and Social Sciences; Immunity; Immunogenicity; Kinases; Leucine; Microorganisms; multidisciplinary; Pattern recognition; Pattern recognition receptors; Plant Diseases - microbiology; Plant Immunity - genetics; Plant species; Prokaryotic Initiation Factors; Proteobacteria - metabolism; Pseudomonas syringae - metabolism; Receptor mechanisms; Receptors; Receptors, Pattern Recognition - genetics; Receptors, Pattern Recognition - metabolism; Science; Science (multidisciplinary); Sensitivity; Translation
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-28887-4

Activation of plant pattern-triggered immunity (PTI) relies on the recognition of microbe-derived structures, termed patterns, through plant-encoded surface-resident pattern recognition receptors (PRRs). We show that proteobacterial translation initiation factor 1 (IF1) triggers PTI in
Arabidopsis thaliana
and related Brassicaceae species. Unlike for most other immunogenic patterns, IF1 elicitor activity cannot be assigned to a small peptide epitope, suggesting that tertiary fold features are required for IF1 receptor activation. We have deployed natural variation in IF1 sensitivity to identify
Arabidopsis
leucine-rich repeat (LRR) receptor-like protein 32 (RLP32) as IF1 receptor using a restriction site-associated DNA sequencing approach. RLP32 confers IF1 sensitivity to
rlp32
mutants, IF1-insensitive
Arabidopsis
accessions and IF1-insensitive
Nicotiana benthamiana
, binds IF1 specifically and forms complexes with LRR receptor kinases SOBIR1 and BAK1 to mediate signaling. Similar to other PRRs, RLP32 confers resistance to
Pseudomonas syringae
, highlighting an unexpectedly complex array of bacterial pattern sensors within a single plant species.
Pattern-triggered immunity is activated by recognition of microbe-derived structures by host pattern recognition receptors. Here the authors use a genotype-by sequencing approach to show that bacterial translation initiation factor 1 triggers PTI in
Arabidopsis thaliana
after recognition by the RLP32 receptor.