Lymphocyte innateness defined by transcriptional states reflects a balance between proliferation and effector functions

• Published in: Nature communications Vol. 10; no. 1; p. 687
• Main Authors: Gutierrez-Arcelus, Maria, Teslovich, Nikola, Mola, Alex R., Polidoro, Rafael B., Nathan, Aparna, Kim, Hyun, Hannes, Susan, Slowikowski, Kamil, Watts, Gerald F. M., Korsunsky, Ilya, Brenner, Michael B., Raychaudhuri, Soumya, Brennan, Patrick J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08-02-2019; Nature Publishing Group; Nature Portfolio
• Subjects: 13/106; 13/21; 13/31; 38/77; 38/91; 631/114; 631/250/1619/554; 631/250/2502; 631/250/2504; Cell proliferation; Cell Proliferation - physiology; Effector cells; Female; Gene expression; Gene Ontology; Heterogeneity; Humanities and Social Sciences; Humans; Immunity, Innate - physiology; Immunophenotyping; Invariants; Leukocytes, Mononuclear - metabolism; Lymphocyte Activation - physiology; Lymphocytes; Lymphocytes - metabolism; Lymphocytes T; Male; Mucosa; multidisciplinary; Natural killer cells; Natural Killer T-Cells - metabolism; Populations; Ribonucleic acid; RNA; Science; Science (multidisciplinary); T-Lymphocyte Subsets - metabolism; Transcription
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-019-08604-4

How innate T cells (ITC), including invariant natural killer T (iNKT) cells, mucosal-associated invariant T (MAIT) cells, and γδ T cells, maintain a poised effector state has been unclear. Here we address this question using low-input and single-cell RNA-seq of human lymphocyte populations. Unbiased transcriptomic analyses uncover a continuous ‘innateness gradient’, with adaptive T cells at one end, followed by MAIT, iNKT, γδ T and natural killer cells at the other end. Single-cell RNA-seq reveals four broad states of innateness, and heterogeneity within canonical innate and adaptive populations. Transcriptional and functional data show that innateness is characterized by pre-formed mRNA encoding effector functions, but impaired proliferation marked by decreased baseline expression of ribosomal genes. Together, our data shed new light on the poised state of ITC, in which innateness is defined by a transcriptionally-orchestrated trade-off between rapid cell growth and rapid effector function.
Innate T cells (ITC) contain many subsets and are poised to promptly respond to antigens and pathogens, but how this poised state is maintained is still unclear. Here the authors perform single-cell RNA-seq to align the various ITC subsets along an ‘innateness gradient’ that is associated with changes in proliferation and effector functions.