Analysis of recombination and gene distribution in the 2L1.0 region of wheat ( Triticum aestivum L.) and barley ( Hordeum vulgare L.)

• Published in: Genomics (San Diego, Calif.) Vol. 86; no. 1; pp. 47 - 54
• Main Authors: Dilbirligi, Muharrem, Erayman, Mustafa, Gill, Kulvinder S.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-07-2005; Elsevier
• Subjects: 2L1.0 region; barley; Biological and medical sciences; Chromosome Mapping; Chromosomes, Plant - genetics; Classical genetics, quantitative genetics, hybrids; Comparative genomics; expressed sequence tags; Fundamental and applied biological sciences. Psychology; gene-rich regions; genes; Genes, Plant - genetics; Genes. Genome; genetic markers; Genetic Markers - genetics; genetic recombination; Genetics of eukaryotes. Biological and molecular evolution; Hordeum - genetics; Hordeum vulgare; Methods, theories and miscellaneous; Molecular and cellular biology; Molecular genetics; physical chromosome mapping; Physical mapping; Recombination; Recombination, Genetic; Synteny; Triticeae; Triticum - genetics; Triticum aestivum; wheat; Comparative genomics; 2L1.0 region; Recombination; Hordeum vulgare; Triticum aestivum; Physical mapping; Genetic mapping; Monocotyledones; Genomics; Gene; Gramineae; Analysis; Angiospermae; Distribution; Spermatophyta; Physical map
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2005.03.008

Both wheat and barley belong to tribe Triticeae and are closely related. High-density detailed comparison of physical and genetic linkage maps revealed that wheat genes are present in physically small gene-rich regions (GRRs). One of the largest GRRs is located around fraction length 1.0 of the long arm of wheat homoeologous group
2 chromosomes termed the “2L1.0 region.” The main objective of this study was to analyze the structural and functional organization of the 2L1.0 region in barley in comparison to wheat. Using the 29 physically mapped RFLP markers for the region, wheat and barley consensus genetic linkage maps of the 2L1.0 region were generated by combining information from 18 wheat and 7 barley genetic linkage maps. Comparative analysis using these consensus maps and other available wheat and barley mapping resources identified 227 DNA markers and ESTs for the region. The region accounted for 58% of the genes and 68% of the arm’s recombination in wheat. However, the corresponding region in barley accounted for about 42% of the genes and 81% of the recombination. The kb/cM ratio for the region was 122 in barley compared to 244 in wheat. Distribution of genes and recombination varied between the two species even though the gene order and density were similar.