Using wintergreen oil for mounting mosquito larvae: a safer alternative to xylene

Permanent mounting of fourth instar mosquito larvae is essential for identifying Aedes spp. This procedure requires extensive exposure to xylene, a clearing agent in the mounting process. We investigated wintergreen oil as a substitute for xylene. Five hundred larvae were mounted on slides to evalua...

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Bibliographic Details
Published in:	Biotechnic & histochemistry Vol. 91; no. 1; p. 63
Main Authors:	Koay, J B, Natasya, N N, Nashithatul, Mag, Ihsanuddin, R, Salleh, F M, Azil, A H
Format:	Journal Article
Language:	English
Published:	England 02-01-2016
Subjects:	Aedes - classification Aedes - drug effects Animals Histocytological Preparation Techniques - methods Larva - classification Larva - drug effects Oils, Volatile - pharmacology Plant Extracts - pharmacology Salicylates - pharmacology Xylenes - chemistry Xylenes - toxicity xylene occupational hazard laboratory technicians mounting methyl salicylate larvae wintergreen oil Aedes dengue shrinkage clearing mosquito
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