NMR studies of a DNA containing 8-hydroxydeoxyguanosine

The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which has an 8-hydroxy-2'-deoxyguanosine (oh8dG) residue at the 4th position. NMR data indicate that the...

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Bibliographic Details
Published in:Nucleic acids research Vol. 19; no. 7; pp. 1407 - 1412
Main Authors: ODA, Y, UESUGI, S, IKEHARA, M, NISHIMURA, S, KAWASE, Y, ISHIKAWA, H, INOUE, H, OHTSUKA, E
Format: Journal Article
Language:English
Published: Oxford Oxford University Press 11-04-1991
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Summary:The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which has an 8-hydroxy-2'-deoxyguanosine (oh8dG) residue at the 4th position. NMR data indicate that the 8-hydroxyguanine (oh8G) base takes a 6,8-diketo tautomeric form and is base-paired to C with Watson-Crick type hydrogen bonds in a B-form structure. The thermal stability of the duplex is reduced, but the overall structure is much the same as that of the unmodified d(CGCGAATTCGCG) duplex. The structural changes caused by 8-hydroxylation of the deoxyguanosine, if any, are localized near the modification site.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.7.1407