The Use of Natural Fiber-Rich Food Product Is Safe and Reduces Aberrant Crypt Foci in a Pre-Clinical Model

• Published in: Nutrients Vol. 13; no. 8; p. 2708
• Main Authors: do Amaral, Luane Aparecida, da Silva Fleming de Almeida, Taina, Oliveira de Souza, Gabriel Henrique, Baranoski, Adrivanio, Souza Maris, Rafael, Bittencourt Junior, Felipe Francisco, Murino Rafacho, Bruna Paola, Duenhas Monreal, Antonio Carlos, Leite Kassuya, Cândida Aparecida, Milan Brochado Antoniolli-Silva, Andréia Conceição, Freitas Dos Santos, Elisvânia, Oliveira, Rodrigo Juliano
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-08-2021; MDPI
• Subjects: 1,2-Dimethylhydrazine; aberrant crypt foci; Aberrant Crypt Foci - chemically induced; Aberrant Crypt Foci - metabolism; Aberrant Crypt Foci - pathology; Aberrant Crypt Foci - prevention & control; Agricultural industry; Animal Feed; Animal models; Animals; Cancer; Carbohydrates; Carcinogenicity; Carcinogens; chemoprevention; Colon - metabolism; Colon - pathology; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - chemically induced; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Colorectal Neoplasms - prevention & control; Cytokines - blood; Dietary fiber; Dietary Fiber - administration & dosage; dietary fibers; Dimethylhydrazines; Disease Models, Animal; Ethylenediaminetetraacetic acid; Food; Food products; functional foods; Genotoxicity; Health services; Humidity; Hydrazine; Inflammation; Inflammation Mediators - blood; Lipids; Male; Microbiota; Ostomy; Prevention; Proteins; Rats; Rats, Wistar; Sensory properties; Brazil; United States; aberrant crypt foci; functional foods; chemoprevention; dietary fibers; 1,2-dimethylhydrazine
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13082708

Colorectal cancer is a highly prevalent disease, requiring effective strategies for prevention and treatment. The present research aimed to formulate a natural fiber-rich food product (NFRFP) and to evaluate its safety, toxicogenetics, and effects on aberrant crypt foci induced by 1,2-dimethyl-hydrazine in a preclinical model.
A total of 78 male Wistar rats were distributed in six experimental groups: negative control, positive control (1,2-Dimethylhydrazine-40 mg/Kg), and four groups fed with 10% NFRFP: NFRFP, pre-treatment protocol, simultaneous treatment, and post-treatment protocol.
The NFRFP was shown to be a good source of fibers and did not change biometric, biochemical, hematological, and inflammatory parameters, and did not induce signs of toxicity and genotoxicity/carcinogenicity. NFRFP exhibited a chemopreventive effect, in all protocols, with damage reduction (% DR) of 75% in the comet test. NFRFP reduced the incidence of aberrant crypt outbreaks by 49.36% in the post-treatment protocol.
The results suggest the applicability of NFRFP in the human diet due to potential production at an industrial scale and easy technological application in different products, since it could be incorporated in food without altering or causing small changes in final product sensory characteristics.