The Effect of Voluntary Exercise on Gut Microbiota in Partially Hydrolyzed Guar Gum Intake Mice under High-Fat Diet Feeding

• Published in: Nutrients Vol. 12; no. 9; p. 2508
• Main Authors: Aoki, Takafumi, Oyanagi, Eri, Watanabe, Chihiro, Kobiki, Nanako, Miura, Suzuka, Yokogawa, Yuka, Kitamura, Hiromi, Teramoto, Fusako, Kremenik, Michael J, Yano, Hiromi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19-08-2020; MDPI
• Subjects: Animals; Bacteria; Bacteroidetes; Body fat; Carbohydrates; Constipation; Diabetes; Diet; Diet, High-Fat - adverse effects; Dietary fiber; Dietary Fiber - administration & dosage; Dietary Fiber - pharmacology; Dietary Supplements; Eating - physiology; Exercise; F/B ratio; Fatty acids; Feces; Firmicutes; Fitness equipment; Food; Galactans - administration & dosage; Galactans - pharmacology; Gastrointestinal Microbiome - drug effects; Glucose; Glucose tolerance; Glucose Tolerance Test; Guar gum; High fat diet; Hydrolysis; Insulin; Insulin Resistance; Intestinal microflora; Male; Mannans - administration & dosage; Mannans - pharmacology; Metabolism; Metabolites; Mice, Inbred C57BL; Microbiota; Nutritional Physiological Phenomena - physiology; Obesity; Obesity - etiology; Obesity - prevention & control; Oxygen Consumption; Physical Conditioning, Animal - physiology; Plant Gums - administration & dosage; Plant Gums - pharmacology; Prebiotics; Running; Wheel running; United States > US; Japan; wheel running; F/B ratio; dietary fiber
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12092508

Although dietary fiber treatment alters the gut microbiota and its metabolite production, it is unclear whether or not exercise habits can have a supplemental effect on changes in gut microbiota in dietary fiber-treated mice. To clarify the supplemental effect of voluntary exercise on gut microbiota in partially hydrolyzed guar gum (PHGG), which is a soluble dietary fiber, treated mice under high-fat diet (HFD) feeding, 4-week-old male C57BL/6J mice (
= 80) were randomly divided into two dietary groups: the control-diet (CD) and HFD. Then, each dietary group was treated with or without PHGG, and with or without wheel running. After the experimental period, measurement of maximal oxygen consumption, a glucose tolerance test and fecal materials collection for analysis of gut microbiota were carried out. Voluntary exercise load in PHGG treatment under HFD feeding showed the supplemental effect of exercise on obesity (
< 0.01) and glucose tolerance (
< 0.01). Additionally, in both CD and HFD groups, voluntary exercise accelerated the decrease in the Firmicutes/Bacteroidetes ratio in mice fed with PHGG (
< 0.01). These findings suggest that voluntary exercise might activate the prevention of obesity and insulin resistance more via change in gut microbiota in mice administrated with PHGG.