MALDI Imaging mass spectrometry: current frontiers and perspectives in pathology research and practice

MALDI Imaging mass spectrometry: current frontiers and perspectives in pathology research and practice

MALDI Imaging mass spectrometry has entered the field of tissue-based research by providing unique advantages for analyzing tissue specimen in an unprecedented detail. A broad spectrum of analytes ranging from proteins, peptides, protein modification over small molecules, drugs and their metabolites...

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Bibliographic Details
Published in:Laboratory investigation Vol. 95; no. 4; pp. 422 - 431
Main Authors: Aichler, Michaela, Walch, Axel
Format: Journal Article
Language:English
Published: New York Elsevier Inc 01-04-2015
Nature Publishing Group US
Nature Publishing Group
Subjects:
14/63
631/1647
82/58
Animals
Biomedical Research
Histological Techniques
Humans
Laboratory Medicine
Medicine
Medicine & Public Health
Mice
Molecular Imaging
pathobiology-in-focus
Pathology
Rats
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Online Access:Get full text
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Summary:MALDI Imaging mass spectrometry has entered the field of tissue-based research by providing unique advantages for analyzing tissue specimen in an unprecedented detail. A broad spectrum of analytes ranging from proteins, peptides, protein modification over small molecules, drugs and their metabolites as well as pharmaceutical components, endogenous cell metabolites, lipids, and other analytes are made accessible by this in situ technique in tissue. Some of them were even not accessible in tissues within the histological context before. Thereby, the great advantage of MALDI Imaging is the correlation of molecular information with traditional histology by keeping the spatial localization information of the analytes after mass spectrometric measurement. This method is label-free and allows multiplex analysis of hundreds to thousands of molecules in the very same tissue section simultaneously. Imaging mass spectrometry brings a new quality of molecular data and links the expert discipline of pathology and deep molecular mass spectrometric analysis to tissue-based research. This review will focus on state-of-the-art of MALDI Imaging mass spectrometry, its recent applications by analyzing tissue specimen and the contributions in understanding the biology of disease as well as its perspectives for pathology research and practice.
ISSN:0023-6837
1530-0307
DOI:10.1038/labinvest.2014.156