Local In Vivo Measures of Muscle Lipid and Oxygen Consumption Change in Response to Combined Vitamin D Repletion and Aerobic Training in Older Adults

• Published in: Nutrients Vol. 11; no. 4; p. 930
• Main Authors: Thomas, D Travis, Schnell, David M, Redzic, Maja, Zhao, Mingjun, Abraha, Hideat, Jones, Danielle, Brim, Howard, Yu, Guoqiang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25-04-2019; MDPI
• Subjects: Adults; Aerobic capacity; Aerobics; Aged; Aging; Body mass; Calciferol; Clinical trials; Deposition; Dietary Supplements; Exercise; Female; Homeostasis; Humans; IMCL; Lipid Metabolism - drug effects; Lipid Metabolism - physiology; Lipids; Male; metabolic function; Metabolism; Middle age; Middle Aged; Muscle, Skeletal - chemistry; Muscle, Skeletal - physiology; Musculoskeletal system; NMR; Nuclear magnetic resonance; Older people; Oxygen consumption; Oxygen Consumption - drug effects; Oxygen Consumption - physiology; Packaging; Physical activity; Physical fitness; Proteins; Sarcopenia; skeletal muscle; Vitamin D; Vitamin D - administration & dosage; Vitamin D - pharmacology; Vitamin deficiency; United States > US; metabolic function; skeletal muscle; vitamin D; IMCL; sarcopenia
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu11040930

Intramyocellular (IMCL), extramyocellular lipid (EMCL), and vitamin D deficiency are associated with muscle metabolic dysfunction. This study compared the change in [IMCL]:[EMCL] following the combined treatment of vitamin D and aerobic training (DAT) compared with vitamin D (D), aerobic training (AT), and control (CTL). Male and female subjects aged 60-80 years with a BMI ranging from 18.5-34.9 and vitamin D status of ≤32 ng/mL (25(OH)D) were recruited to randomized, prospective clinical trial double-blinded for supplement with a 2 × 2 factorial design. Cholecalciferol (Vitamin D
) (10,000 IU × 5 days/week) or placebo was provided for 13 weeks and treadmill aerobic training during week 13. Gastrocnemius IMCL and EMCL were measured with magnetic resonance spectroscopy (MRS) and MRI. Hybrid near-infrared diffuse correlation spectroscopy measured hemodynamics. Group differences in IMCL were observed when controlling for baseline IMCL (
= 0.049). DAT was the only group to reduce IMCL from baseline, while a mean increase was observed in all other groups combined (
= 0.008). IMCL reduction and the corresponding increase in rVO
at study end (
= 0.011) were unique to DAT. Vitamin D, when combined with exercise, may potentiate the metabolic benefits of exercise by reducing IMCL and increasing tissue-level VO
in healthy, older adults.