Redox regulation of the MED28 and MED32 mediator subunits is important for development and senescence

• Published in: Protoplasma Vol. 253; no. 3; pp. 957 - 963
• Main Authors: Shaikhali, Jehad, Céline Davoine, Stefan Björklund, Gunnar Wingsle
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-05-2016; Springer Nature B.V
• Subjects: Arabidopsis; Arabidopsis - drug effects; Arabidopsis - physiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Biomedical and Life Sciences; Botanik; Botany; Cell Biology; Cellbiologi; disulfide bonds; DNA-directed RNA polymerase; Gene Expression Regulation, Plant; homeostasis; Hydrogen Peroxide - pharmacology; Life Sciences; Mediator Complex - genetics; Mediator Complex - metabolism; metabolism; mutants; Mutation; Oxidation-Reduction; phenotype; Plant Roots - drug effects; Plant Roots - genetics; Plant Roots - growth & development; Plant Sciences; Short Communication; transcription factors; Zoology; Redox; Senescence; Mediator; Oligomerization; Root development
• ISSN: 0033-183X; 1615-6102; 1615-6102
• DOI: 10.1007/s00709-015-0853-y

Mediator is a conserved multi-protein complex that acts as a bridge between promoter-bound transcriptional regulators and RNA polymerase II. While redox signaling is important in adjusting plant metabolism and development, the involvement of Mediator in redox homeostasis and regulation only recently started to emerge. Our previous results show that the MED10a, MED28, and MED32 Mediator subunits form various types of covalent oligomers linked by intermolecular disulfide bonds in vitro. To link that with biological significance we have characterized Arabidopsis med32 and med28 mutants and found that they are affected in root development and senescence, phenotypes possibly associated to redox changes.