Distribution and Application of ELISA for the Seroprevalence of Tick-Borne Diseases in Central Equatoria State, Sudan

• Published in: Transboundary and emerging diseases Vol. 55; no. 5-6; pp. 257 - 262
• Main Authors: Salih, D.A, Hassan, S.M, Julla, I.I, Kyule, M.N, Zessin, K-H, El Hussein, A.M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-08-2008; Blackwell Publishing Ltd; Hindawi Limited
• Subjects: Anaplasma marginale; Anaplasma marginale - immunology; anaplasmosis; animal age; Animal diseases; animal pathogens; Animals; Antibodies, Bacterial - blood; Antibodies, Protozoan - blood; antigens; Babesia - immunology; Babesia bigemina; babesiosis; Blood tests; Cattle; Cattle Diseases - blood; Cattle Diseases - epidemiology; disease prevalence; ELISA; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Epidemiology; geographical variation; Parasites; seasonal variation; Seasons; Seroepidemiologic Studies; seroprevalence; Southern Sudan; Sudan - epidemiology; Theileria - immunology; Theileria mutans; Theileria parva; Theileria parva - immunology; theileriosis; Tick-Borne Diseases - blood; Tick-Borne Diseases - epidemiology; Tick-Borne Diseases - veterinary; Sudan
• ISSN: 1865-1682; 1865-1674; 1865-1682
• DOI: 10.1111/j.1865-1682.2008.01032.x

This study was carried out to estimate the prevalence of Theileria parva, Theileria mutans, Babesia bigemina and Anaplasma marginale antibodies among naturally infected cattle in Central Equatoria State, Southern Sudan using indirect enzyme-linked immunosorbent assay (ELISA). Three locations (Khor Rumla, Nyaing and Gumbo) were selected and surveyed every 3 months during the period from January to December 2005. Six cattle herds in the three locations were visited four times during the study period. During each visit, 150 serum samples were collected from indigenous cattle of both sex and of different age groups. Assessment of antibodies against tick-borne diseases was carried out using indirect ELISA. The overall seroprevalence rates of T. parva, T. mutans, B. bigemina and A. marginale were found to be 58.2%, 88.9%, 51.1% and 37.8% respectively. The highest seroprevalence of T. parva was observed in Gumbo (69%). An overall seroprevalence of 43.3% for T. parva was reported in July which was significantly lower than other seasons. Older animals showed higher seroprevalence than younger ones. The implications of these results on the epidemiology of tick-borne diseases are discussed with emphasis on East Coast fever.