Study of the Lipid Profile of ATCC and Clinical Strains of Staphylococcus aureus in Relation to Their Antibiotic Resistance

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 7; p. 1276
• Main Authors: Bisignano, Carlo, Ginestra, Giovanna, Smeriglio, Antonella, La Camera, Erminia, Crisafi, Giuseppe, Franchina, Flavio A, Tranchida, Peter Q, Alibrandi, Angela, Trombetta, Domenico, Mondello, Luigi, Mandalari, Giuseppina
• Format: Journal Article Web Resource
• Language: English
• Published: Switzerland MDPI AG 02-04-2019; MDPI
• Subjects: Abundance; American Type Culture Collection; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Antimicrobial agents; ATCC strains; Bacteria; Biofilms; Cell culture; Cell surface; Chain branching; Chromatography; Chromatography, Gas; Clinical strains; Conjunctiva; Correlation; Drug resistance; Drug Resistance, Microbial; E coli; Fatty acids; Fatty Acids - metabolism; Gram-negative bacteria; Hydrophobic and Hydrophilic Interactions; Hydrophobicity; Life sciences; Lipid composition; Lipids; Mass spectrometry; Microbial Sensitivity Tests; Palmitic acid; Phospholipids - metabolism; Prostheses; Prosthetics; Sciences du vivant; Scientific imaging; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - metabolism; Staphylococcus infections; Statistical analysis; Strains (organisms); Taxonomy; Ulcers; hydrophobicity; lipids; antibiotic resistance; clinical strains; ATCC strains; Staphylococcus aureus
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24071276

A number of reports have indicated a relationship between bacterial resistance to antibiotics and their lipid composition. In the present study, we characterized the lipid profiles of American Type Culture Collection (ATCC) and clinical strains of
and its correlation with antibiotic resistance and hydrophobicity. The following strains were used:
ATCC 6538P,
ATCC 43300 (MRSA), seven clinical strains from the pharynges, two strains from duodenal ulcers, four strains from hip prostheses, and one strain from the conjunctiva. Lipid-related differentiation was observed across the
strains: the higher abundance of
-pentadecanoic acid (
-C
) and
-heptadecanoic acid (
-C
), followed by
-pentadecanoic acid (
-C
), suggested that these were common lipids.
-tridecanoic acid (
-C
) and
-tridecanoic acid (
-C
),
-hexadecanoic acid (
-C
) and
-hexadecanoic acid (
-C
), and all forms of octadecanoic acid (C
) were usually detected in low abundance. Strains isolated from pharynges showed the highest ratio of branched/straight chains. A distinction in two clusters based on the amount and type of bacterial lipids identified was obtained, which correlated to the antibiotic resistance, the strains origin, and the cell-surface hydrophobicity. We report a potential correlation between the lipid profile of
strains, site of infection, antibiotic resistance, and cell-surface hydrophobicity. These results, which still need further insights, could be a first step to identifying antibiotic resistance in response to environmental adaptation.