Molecular characterization of a salt-tolerant bacterial community in the rice rhizosphere

• Published in: Research in microbiology Vol. 153; no. 9; pp. 579 - 584
• Main Authors: Tripathi, Anil K., Verma, Subhash C., Ron, Eliora Z.
• Format: Journal Article
• Language: English
• Published: Paris Elsevier SAS 01-11-2002; Elsevier
• Subjects: Alcaligenes - metabolism; Alcaligenes xylosoxidans; Animal, plant and microbial ecology; ARDRA; Biological and medical sciences; Cluster Analysis; DNA Fingerprinting; DNA Restriction Enzymes - chemistry; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Freshwater; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacteria - genetics; Gram-Negative Bacteria - metabolism; Human pathogens; Microbial ecology; Microflora of plants; Ochrobactrum anthropi; Ochrobactrum anthropi - metabolism; Oryza - microbiology; Oryza sativa; Plant Roots - microbiology; Polymerase Chain Reaction; Pseudomonas aeruginosa; Pseudomonas aeruginosa - metabolism; Random Amplified Polymorphic DNA Technique; Rice rhizosphere; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics; Salt-tolerant bacteria; Sequence Analysis, DNA; Serratia marcescens; Serratia marcescens - metabolism; Sodium Chloride - metabolism; India; Salt-tolerant bacteria; ARDRA; Rice rhizosphere; Human pathogens; Oryza sativa; Rhizosphere; Monocotyledones; Salt resistance; Gramineae; Angiospermae; Genotype; Spermatophyta; Genetic diversity; Microbial community
• ISSN: 0923-2508; 1769-7123
• DOI: 10.1016/S0923-2508(02)01371-2

The diversity of salt-tolerant bacteria present in the rhizosphere of
Oryza sativa was investigated. Fourteen bacterial strains, isolated after enrichment in nitrogen-free, semi-solid medium and showing tolerance to 3% NaCl, were analyzed by restriction patterns produced by amplified DNA coding for 16S rDNA (ARDRA) with enzymes
Sau3AI,
AluI and
RsaI which showed that they were represented by 4 ARDRA types. Biodiversity among the 14 strains was also analyzed by the random amplified polymorphic DNA (RAPD) technique with a 10-mer primer. Partial nucleotide sequence of 16S rDNA assigned these clusters to
Serratia marcescens,
Pseudomonas aeruginosa,
Alcaligenes xylosoxidans and
Ochrobactrum anthropi. Notably, all four bacterial species are potential human pathogens that infect immunocompromised patients.