Directs effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on adaptive immunogenesis

• Published in: Human vaccines & immunotherapeutics Vol. 15; no. 12; pp. 2903 - 2909
• Main Authors: Seledtsov, Victor Ivanovich, Malashchenko, Vladimir V., Gazatova, Natalja D., Meniailo, Maksim E., Morozova, Ekaterina M., Seledtsova, Galina V.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 02-12-2019; Taylor & Francis Group
• Subjects: Adaptive Immunity - drug effects; Cells, Cultured; cytokines; Cytokines - immunology; Granulocyte-macrophage colony-stimulating factor; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology; Humans; Lipopolysaccharides; Lymphocyte Activation - drug effects; Macrophages - drug effects; Macrophages - immunology; monocyte/macrophage; Monocytes - drug effects; Monocytes - immunology; Phenotype; Research Paper; T-cell subsets; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; СD expression; cytokines; СD expression; monocyte/macrophage; Granulocyte-macrophage colony-stimulating factor; T-cell subsets
• ISSN: 2164-5515; 2164-554X
• DOI: 10.1080/21645515.2019.1614396

Background: We studied direct effects of human granulocyte-macrophage colony stimulating factor (GM-CSF) on phenotypical characteristics and cytokine-production of non-activated and activated human monocytes/macrophages (Mc/Mphs) and T cells.
Methods: Purified Mc/Mphs were activated by bacterial lipopolysaccharide (LPS, 1 μg/ml) for 24 h, while T cells were activated by particles conjugated and antibodies (Abs) against human CD2, CD3, and CD28 for 48 h.
Results: GM-CSF treatment (0.01-10 ng/ml) was shown to reduce percentages of CD197 (CCR7)-positive cells in non-activated Mph cultures, without affecting significantly CD14
+
(LPS co-receptor), CD16
+
(FcγRIII, low-affinity Fc-receptor), CD119
+
(interferon-gamma receptor 1), and CD124
+
(IL4 receptor α-subunit) cells. In addition, GM-CSF reduced relative numbers of CD197
+
cells, as well as CD14
+
, CD16
+
, and CD119
+
cells in activated Mph cultures without affecting CD124
+
cell distribution. GM-CSF at the highest dose of 10 ng/ml enhanced TNF-α and IL-6 (but not IL-1β and IL-10) production in activated Mc/Mphs. In activated T cell cultures, GM-CSF at 0.1-1.0 ng/ml augmented CD38
+
cell numbers in naïve СD45RA
+
/СD197
+
and central memory СD45RA
−
/СD197
+
cell subsets, with no effect on effector СD45RA
−
/СD197
−
and terminally differentiated effector СD45RA
+
/СD197
−
cells. GM-CSF at a low dose (0.01 ng/ml) down-regulated INF-γ production, while at a high dosage (10.0 ng/ml) up-regulated IL-2 and IL-4 production.
Conclusion: In general, the results suggest that GM-CSF is able to facilitate the implication of both Mph and T cells in the adaptive immunogenesis.