Leporinus elongatus (Characiformes, Anostomidae): complete mtDNA sequence of an economically important fish from the Paraná and La Plata river basins

• Published in: Mitochondrial DNA. Part B. Resources Vol. 2; no. 1; pp. 261 - 263
• Main Authors: Bedore, Alessandra Gomes, Resende, Leonardo Cardoso, Carmo, Anderson Oliveira do, Nuñez Rodriguez, Daniela, Martins, Ana Paula Vimieiro, Kalapothakis, Evanguedes
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-01-2017; Taylor & Francis Group
• Subjects: Mitochondrial genome; Mitogenome Announcement; next-generation sequencing; piapara; piau verdadeiro; piau verdadeiro; piapara; next-generation sequencing; Mitochondrial genome
• ISSN: 2380-2359; 2380-2359
• DOI: 10.1080/23802359.2017.1318685

Leporinus elongatus is an important commercial fish found in the La Plata and Paraná River basins. Next-generation sequencing was used to sequence the mitochondrial DNA (mtDNA) of L. elongatus. The mtDNA was assembled using the CLC Workbench software v. 9.0 and subsequently aligned to other 10 complete fish mitochondrial sequences to enable phylogenetic analysis using MEGA 7.0. The complete mtDNA molecule had 16,784 bp and its GC content was 43%. The mtDNA structure was similar to that of other vertebrates: two ribosomal RNA, 22 transfer RNA, 13 protein-coding genes, and a D-loop region containing 1115 bp. Phylogenetic analysis yielded a tree with high bootstrap value that was coherent with the current phylogeny proposed for Characiformes.