Distribution of unlinked receptor sites for transposed Ac elements from the bz-m2(Ac) allele in maize

• Published in: Genetics (Austin) Vol. 136; no. 1; pp. 261 - 279
• Main Authors: Dooner, H.K, Belachew, A, Burgess, D, Harding, S, Ralston, M, Ralston, E
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Genetics Soc America 01-01-1994; Genetics Society of America
• Subjects: Alleles; Biological and medical sciences; CHROMOSOME; Chromosome Mapping; Classical genetics, quantitative genetics, hybrids; Corn; CROMOSOMAS; Crosses, Genetic; DNA Transposable Elements; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Plant; Genetic Linkage; GENETICA; Genetics; Genetics of eukaryotes. Biological and molecular evolution; GENETIQUE; Investigations; LOCALISATION DE GENE; LOCALIZACION DE GENES; LOCI; LOCUS; Models, Genetic; Pteridophyta, spermatophyta; SEGREGACION; SEGREGATION; TRANSLOCACION DE CROMOSOMAS; TRANSLOCATION CHROMOSOMIQUE; Translocation, Genetic; Vegetals; ZEA MAYS; Zea mays - genetics; Genetic mapping; Transposition; Monocotyledones; Zea mays; Gramineae; Chromosome 9; Angiospermae; Spermatophyta; Transposable element; Mutation; Ac element
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/136.1.261

We have shown before that the Ac element from the maize bz-m2(Ac) allele,located in the short arm of chromosome 9 (9S), transposes preferentially to sites that are linked to the bz donor locus. Yet, about half of the Ac transpositions recovered from bz-m2(Ac) are in receptor sites not linked to the donor locus. In this study, we have analyzed the distribution of those unlinked receptor sites. Thirty-seven transposed Ac (trAc) elements that recombined independently of the bz locus were mapped using a set of wx reciprocal translocations. We found that the distribution of unlinked receptor sites for trAs was not random. Ten trAcs mapped to 9L, i.e., Ac had transposed to sites physically, if not genetically, linked to the donor site. Among chromosomes other than 9, the Ac element of bz-m2(Ac) appeared to have transposed preferentially to certain chromosomes, such as 5 and 7, but infrequently to others, such as 1, the longest chromosome in the maize genome. The seven trAc elements in chromosome 5 were mapped relative to markers in 5S and 5L and localized to both arms of 5. We also investigated the transposition of Ac to the homolog of the donor chromosome. We found that Ac rarely transposes from bz-m2(Ac) to the homologous chromosome 9. The clustering of Ac receptor sites around the donor locus has been taken to mean that a physical association between the donor site and nearby receptor sites occurs during transposition. The preferential occurrence of 9L among chromosomes harboring unlinked receptor sites would be expected according to this model, since sites in 9L would tend to be physically closer to 9S than sites in other chromosomes. The nonrandom pattern seen among the remaining chromosomes could reflect an underlying nuclear architecture, i.e., an ordering of the chromosomes in the interphase nucleus, as suggested from previous cytological observations