A probiotic strain of Escherichia coli, Nissle 1917, given orally exerts local and systemic anti‐inflammatory effects in lipopolysaccharide‐induced sepsis in mice

• Published in: British journal of pharmacology Vol. 157; no. 6; pp. 1024 - 1033
• Main Authors: Arribas, B, Rodríguez‐Cabezas, ME, Camuesco, D, Comalada, M, Bailón, E, Utrilla, P, Nieto, A, Concha, A, Zarzuelo, A, Gálvez, J
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-07-2009; Nature Publishing Group
• Subjects: Administration, Oral; Animals; Anti-Inflammatory Agents, Non-Steroidal - administration & dosage; Bacterial diseases; Bacterial sepsis; Biological and medical sciences; Cells, Cultured; cytokines; Escherichia coli; Escherichia coli Nissle 1917; Female; Human bacterial diseases; IgG; Infectious diseases; Inflammation Mediators - administration & dosage; Inflammation Mediators - antagonists & inhibitors; Inflammation Mediators - toxicity; intestinal anti‐inflammatory activity; Lipopolysaccharides - antagonists & inhibitors; Lipopolysaccharides - toxicity; Male; Medical sciences; Mice; mice LPS septic shock; Mice, Inbred BALB C; Pharmacology. Drug treatments; Probiotics - administration & dosage; Rats; Rats, Wistar; Research Papers; Shock, Septic - chemically induced; Shock, Septic - pathology; Shock, Septic - prevention & control; TNBS rat colitis; Shock; mice LPS septic shock; Rat; Escherichia coli; Escherichia coli Nissle 1917; IgG; Sepsis syndrome; intestinal anti-inflammatory activity; Lipopolysaccharide; Bacteria; Intestinal disease; cytokines; Enterobacteriaceae; Probiotic; Digestive system; Septicemia; Rodentia; Antiinflammatory agent; Cytokine; Gut; Oral administration; Infection; Vertebrata; Mammalia; Mouse; Animal; Digestive diseases; Colitis; TNBS rat colitis
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1111/j.1476-5381.2009.00270.x

Background and purpose:  Escherichia coli Nissle 1917 is a probiotic strain used in the treatment of intestinal immune diseases, including ulcerative colitis. The aim of the present study was to test if this probiotic bacterium can also show systemic immunomodulatory properties after oral administration.
Experimental approach:  The probiotic strain was administered to rats or mice for 2 weeks before its assay in two experimental models of altered immune response, the trinitrobenzenesulphonic acid (TNBS) model of rat colitis, localized in the colon, and the lipopolysaccharide (LPS) model of systemic septic shock in mice. Inflammatory status was evaluated both macroscopically and biochemically after 1 week in the TNBS model or after 24 h in the LPS shock model. In addition, splenocytes were obtained from mice and stimulated, ex vivo, with concanavalin A or LPS to activate T or B cells, respectively, and cytokine production (IL‐2, IL‐5 and IL‐10) by T cells and IgG secretion by B cells measured.
Key results:  E. coli Nissle 1917 was anti‐inflammatory in both models of altered immune response. This included a reduction in the pro‐inflammatory cytokine tumour necrosis factor‐α both in the intestine from colitic rats, and in plasma and lungs in mice treated with LPS. The systemic beneficial effect was associated with inhibited production of the T cell cytokines and by down‐regulation of IgG release from splenocyte‐derived B cells.
Conclusions and implications:  The anti‐inflammatory effects of E. coli Nissle 1917 given orally were not restricted to the gastrointestinal tract.