Intranasal Immunization with Yersinia enterocolitica O:8 Cellular Extract Protects against Local Challenge Infection

• Published in: MICROBIOLOGY and IMMUNOLOGY Vol. 42; no. 11; pp. 781 - 788
• Main Authors: Genaro, María Silvia Di, Escudero, María Esther, Muñoz, Estela, Aguilera, Claudia, Scardapane, Luis, De Guzmán, Ana María Stefanini
• Format: Journal Article
• Language: English
• Published: Tokyo Blackwell Publishing Ltd 01-01-1998; Center For Academic Publications Japan; Center for Academic Publications Japan
• Subjects: Administration, Intranasal; Animals; Antibodies, Bacterial - biosynthesis; Bacterial Vaccines - administration & dosage; Bacterial Vaccines - immunology; Bacteriology; Biological and medical sciences; Cholera Toxin - immunology; Female; Fundamental and applied biological sciences. Psychology; Immunity, Mucosal; Immunization; Intranasal; Lung - microbiology; Male; Mice; Microbiology; Protection; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Yersinia enterocolitica; Yersinia enterocolitica - immunology; Yersinia Infections - prevention & control; Immunoprotection; IgA; Mucosa; Lung; Rodentia; Cell extract; Vaccine; Intranasal administration; Respiratory system; Yersinia enterocolitica; Vertebrata; Local immunity; Mammalia; Immunogenicity; Mouse; Bacteria; Humoral immunity; Enterobacteriaceae
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1998.tb02352.x

Yersinia enterocolitica is enteropathogenic for humans and rodents. Immune protection from oral and respiratory pathogens may be most effectively elicited following intranasal (i.n.) vaccination. An experimental murine intranasal challenge model was used to evaluate the immunogenicity of a Y. enterocolitica O:8 cellular extract (CE) in mucosa. This antigenic preparation has demonstrated to induce protection by subcutaneous immunization. Mice were immunized intranasally with two doses of CE. Immunized and non-immunized animals were challenged with 5×106 colony-forming units (CFU) by nasal infection. Antibodies in serum and bronchoalveolar lavage (b.a.l.) fluid were assessed before and 48hr after challenge. The CFU were determined by analysis of lung homogenate samples. The CE immunization induced significant b.a.l.-specific IgA and IgG, and serum-specific IgG, IgA and IgM. Histopathological studies 24 and 48hr postchallenge demonstrated that immunization protected against progressive lesions resulting from Y. enterocolitica invasion of the pulmonary mucosa. The CFU in the lungs showed that CE immunization led to significant clearance as compared to the bacterial level in nonimmunized controls. From the results obtained, it can be concluded that CE can induce local and systemic immunity and protect against nasal infection.