The effect of TAT conjugated platinum nanoparticles on lifespan in a nematode Caenorhabditis elegans model

• Published in: Biomaterials Vol. 31; no. 22; pp. 5849 - 5854
• Main Authors: Kim, Juewon, Shirasawa, Takuji, Miyamoto, Yusei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01-08-2010
• Subjects: Advanced Basic Science; Animals; Antioxidants - chemistry; Antioxidants - pharmacokinetics; Antioxidants - pharmacology; Biomimetic material; Biomimetic Materials - chemistry; Biomimetic Materials - pharmacokinetics; Biomimetic Materials - pharmacology; Caenorhabditis elegans; Caenorhabditis elegans - metabolism; Dentistry; Drug Carriers - chemistry; Drug delivery; HIV-1 - chemistry; Human immunodeficiency virus 1; Metal surface treatment; Nano particle; Nanoparticles - chemistry; Nematoda; Oxidative Stress - drug effects; Platinum; Platinum - chemistry; Platinum - pharmacokinetics; Platinum - pharmacology; Surface modification; Surface Properties; tat Gene Products, Human Immunodeficiency Virus - chemistry; Nano particle; Metal surface treatment; Surface modification; Drug delivery; Platinum; Biomimetic material
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2010.03.077

Abstract We have shown that platinum nanoparticle species (nano-Pt) is a superoxide dismutase/catalase mimetic that scavenges superoxide and hydrogen peroxide. In Caenorhabditis elegans , nano-Pt functions as an effective antioxidant that induces an extension in lifespan and strong resistance against excessive oxidative stress. Our study with C. elegans was the first trial to use nano-Pt as a bio-active substance. However, a high concentration of nano-Pt was required for these survival effects, probably due to limited membrane permeability. Here, we show that the conjugation of nano-Pt with an HIV-1 TAT fusion protein C-terminally linked to a peptide with high affinity for platinum improves internalization, eliciting a similar level of antioxidant effects at one hundredth the concentration of unconjugated nano-Pt. This approach is a potential method to facilitate translocation of bio-active nanoparticles into living organisms and could be a model assay for estimate the effects of antioxidant in living organism.