Magnesium Sulfate Reduces Carrageenan-Induced Rat Paw Inflammatory Edema Via Nitric Oxide Production

• Published in: Dose-response Vol. 21; no. 1; p. 15593258231155788
• Main Authors: Srebro, Dragana, Dožić, Branko, Savić Vujović, Katarina, Medić Brkić, Branislava, Vučković, Sonja
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01-01-2023; SAGE PUBLICATIONS, INC; SAGE Publishing
• Subjects: Drug dosages; Edema; Nitric oxide; Special Collection: Inflammatory Parameters as Early Biomarkers in Prediction and Monitoring of Therapeutic Effects in Preclinical and Clinical Studies; preemptive therapy; anti-inflammatory; emptive therapy; magnesium; anti-edematous; NOS inhibitors
• ISSN: 1559-3258; 1559-3258
• DOI: 10.1177/15593258231155788

Background
Magnesium is an antagonist of the N-methyl-D-aspartate receptor. This study aimed to investigate the anti-edematous effect of magnesium sulfate (MS) in different protocols of use and the possible mechanism of its action.
Methods
In a rat model of carrageenan-induced paw inflammation, the anti-edematous activity of MS was assessed with a plethysmometer. The effects of the nonselective inhibitor (L-NAME), selective inhibitor of neuronal (L-NPA) and inducible (SMT) nitric oxide synthase on the effects of MS were evaluated.
Results
MS administered systemically before or after inflammation reduced edema by 30% (5 mg/kg, P < .05) and 55% (30 mg/kg, P < .05). MS administered locally (.5 mg/paw, P < .05) significantly prevented the development of inflammatory edema by 60%. L-NAME, intraperitoneally administered before MS, potentiated (5 mg/kg, P < .05) or reduced (3 mg/kg, P < .05), while in the highest tested dose L-NPA (2 mg/kg, P < .01) and SMT (.015 mg/kg, P < .01) reduced the anti-edematous effect of MS.
Conclusions
Magnesium is a more effective anti-edematous drug in therapy than for preventing inflammatory edema. The effect of MS is achieved after systemic and local peripheral administration and when MS is administered as a single drug in a single dose. This effect is mediated at least in part via the production of nitric oxide.