impact of ultraviolet-B radiation on the motility of the freshwater epipelic diatom Nitzschia lineariz

• Published in: Global change biology Vol. 5; no. 2; pp. 191 - 199
• Main Authors: Moroz, A.L, Ehrman, J.M, Clair, T.A, Gordon, R.J, Kaczmarska, I
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-02-1999
• Subjects: behavior; epipelic diatoms; motility; movement; Nitzschia; Nitzschia lineariz; solar radiation; ultraviolet radiation; UV-B; New Brunswick
• ISSN: 1354-1013; 1365-2486
• DOI: 10.1046/j.1365-2486.1999.00225.x

Depletion of the stratospheric ozone layer has been of increased concern due to correlated increases in ambient ultraviolet radiation. Our research investigated the diel response of the motile behaviour of Nitzschia lineariz in both natural (freshwater stream) and experimental conditions. We classified the behaviour of individual diatom cells as gliding, immobile and oscillating. The experimental conditions were of simultaneous exposure either to net ambient solar radiation (control) or to ambient plus enhanced UV‐B levels (treatment). UV‐B for the control condition was filtered out using polyester filters; cells exposed to UV‐B were irradiated through cellulose diacetate filters. UVB‐313 levels were augmented to 33% (290 mW m–2) and 66% (365 mW m–2) above the local average ambient levels (220 mW m–2) for 10 h. The mobile behaviour of cells was examined and quantified every 2 consecutive hours from dawn to dusk in subsampled populations. The number of gliding cells in subsample populations declined on overcast days at both the 33 and 66% UV‐B enhancement levels, whereas the number of immobile and oscillating cells increased with increased UV‐B exposure. On sunny days, mobile behaviour was not affected at either enhancement level, which suggests that cellular repair mechanisms may be activated during sunny conditions.