The Roles of Angiotensin II in Stretched Periodontal Ligament Cells

• Published in: Journal of dental research Vol. 90; no. 2; pp. 181 - 185
• Main Authors: Monnouchi, S., Maeda, H., Fujii, S., Tomokiyo, A., Kono, K., Akamine, A.
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01-02-2011; International Association for Dental Research; SAGE PUBLICATIONS, INC
• Subjects: Adult; Alkaline Phosphatase - biosynthesis; Alkaline Phosphatase - genetics; Angiotensin II - pharmacology; Angiotensin II - physiology; Angiotensinogen - biosynthesis; Angiotensinogen - genetics; Animals; Biological and medical sciences; Cells, Cultured; Dental Stress Analysis; Dentistry; Female; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression; Humans; Male; Mastication; Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus; Osteoprotegerin - biosynthesis; Periodontal Ligament - cytology; Periodontal Ligament - metabolism; RANK Ligand - biosynthesis; Rats; Recombinant Proteins - pharmacology; Stress, Mechanical; Transforming Growth Factor beta1 - biosynthesis; Transforming Growth Factor beta1 - genetics; Up-Regulation; Vertebrates: digestive system; stretch; angiotensin II; human periodontal ligament fibroblastic cells; Human; Alkaline phosphatase; Enzyme; Transforming growth factor β; Phosphoric monoester hydrolases; Esterases; Prohormone; Biomechanics; Renin angiotensin system; Periodontal ligament; Mechanical stress; Animal; Angiotensinogen; Hydrolases; Angiotensin II; Fibroblast
• ISSN: 0022-0345; 1544-0591
• DOI: 10.1177/0022034510382118

The loading caused by occlusion and mastication plays an important role in maintaining periodontal ligament (PDL) tissues. We hypothesized that a loading magnitude would be involved in the production of biological factors that function in the maintenance of PDL tissues. Here, we identified up-regulated gene expressions of transforming growth factor-β1 (TGF-β1), alkaline phosphatase (ALP), and angiotensinogen in human PDL fibroblastic cells (HPLFs) that were exposed to 8% stretch loading. Immunolocalization of angiotensin I/II (Ang I/II), which was converted from angiotensinogen, was detected in rat PDL tissues. HPLFs that were stimulated by Ang II also increased their gene expressions of TGF-β1 and ALP. Furthermore, the antagonist for Ang II type 2 receptor, rather than for type 1, significantly inhibited gene expressions induced by the stretch loading. Analysis of these data suggests that Ang II mediates the loading signal in stretched HPLFs to induce expressions of TGF-β1 and ALP.