Production of reducing sugar from Enteromorpha intestinalis by hydrothermal and enzymatic hydrolysis

• Published in: Bioresource technology Vol. 161; pp. 348 - 353
• Main Authors: Kim, Dong-Hyun, Lee, Sang-Bum, Jeong, Gwi-Taek
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-06-2014; Elsevier
• Subjects: Bioenergy; Biofuel production; Biofuels; Biological and medical sciences; Biomass; Biotechnology; Carbohydrate Metabolism; Carbohydrates - chemistry; Carbohydrates - isolation & purification; Chlorophyta - chemistry; Chlorophyta - metabolism; Effectiveness; Energy; Enteromorpha intestinalis; Enzymatic hydrolysis; Fundamental and applied biological sciences. Psychology; Hydrolysis; Hydrothermal treatment; Industrial applications and implications. Economical aspects; Marine; Reaction time; Renewable energy; Severity factor; Sugars; Temperature; Severity factor; Enteromorpha intestinalis; Bioenergy; Hydrothermal treatment; Enzymatic hydrolysis; Edible alga; Hydrolysis; Reducing sugar; Chlorophyta; Ulvophyceae; Algae; Enzymatic digestion
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.03.078

•Marine green-algae Enteromorpha intestinalis converted to reducing sugar.•7.3g/L TRS obtained under hydrothermal condition (1:10 S/L ratio, 170°C, 60min).•By post-hydrothermal enzymatic hydrolysis, 20.1g/L yield was achieved.•The higher severity factor can cause the over-degradation of carbohydrates.
In this work, to evaluate the efficacy of marine macro-algae Enteromorpha intestinalis as a potential bioenergy resource, the effects of reaction conditions (solid-to-liquid ratio, reaction temperature, and reaction time) on sugars produced by a combined process of hydrothermal and enzymatic hydrolysis were investigated. As a result of the hydrothermal hydrolysis, a 7.3g/L (8% yield) total reducing sugar was obtained under conditions including solid-to-liquid ratio of 1:10, reaction temperature of 170°C, and reaction time of 60min. By subsequent (post-hydrothermal) enzymatic hydrolysis of samples treated at 170°C for 30min, a 20.1g/L (22% yield) was achieved.