late blight resistance locus Rpi-blb3 from solanum bulbocastanum belongs to a major late blight R gene cluster on chromosome 4 of potato

• Published in: Molecular plant-microbe interactions Vol. 18; no. 7; pp. 722 - 729
• Main Authors: Park, T.-H, Gros, J, Sikkema, A, Vleeshouwers, G.A.A, Muskens, M, Allefs, S, Jacobsen, E, Vissar, R.G.F, Vossen, E.A.G. van der
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01-07-2005; American Phytopathological Society; The American Phytopathological Society
• Subjects: aflp markers; BAC library; Biological and medical sciences; broad-spectrum resistance; bulked segregant analysis; chromosome mapping; chromosome walking; disease resistance; Fundamental and applied biological sciences. Psychology; fungal diseases of plants; Fungal plant pathogens; high-resolution map; marker-assisted selection; molecular sequence data; multigene family; nematode globodera-rostochiensis; nucleotide sequences; Phytopathology. Animal pests. Plant and forest protection; Phytophthora infestans; phytophthora-infestans mont; plant genetics; plant pathogenic fungi; potatoes; quantitatively-inherited resistance; race-specific resistance; Solanum bulbocastanum; Solanum tuberosum; synteny; virus-x; wild plants; Plant pathogen; Microbiology; bulked segregant analysis; chromosome walking; Genomics; Phycomycetes; Identification; Fungi; Dicotyledones; Angiospermae; Phytophthora infestans; Genetics; Multigene family; Solanaceae; Thallophyta; Gene cluster; Solanum tuberosum; Chromosome; BAC library; synteny; Amplification; Resistance; Spermatophyta; Tuber plant; Cultivar; Polymorphism
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/mpmi-18-0722

Late blight, caused by Phytophthora infestans, is one of the most devastating diseases in cultivated potato. Breeding of new potato cultivars with high levels of resistance to P. infestans is considered the most durable strategy for future potato cultivation. In this study, we report the identification of a new late-blight resistance (R) locus from the wild potato species Solanum bulbocastanum. Using several different approaches, a high-resolution genetic map of the new locus was generated, delimiting Rpi-blb3 to a 0.93 cM interval on chromosome 4. One amplification fragment length polymorphism marker was identified that cosegregated in 1,396 progeny plants of an intraspecific mapping population with Rpi-blb3. For comparative genomics purposes, markers linked to Rpi-blb3 were tested in mapping populations used to map the three other late-blight R loci Rpi-abpt, R2, and R2-like also to chromosome 4. Marker order and allelic conservation suggest that Rpi-blb3, Rpi-abpt, R2, and R2-like reside in the same R gene cluster on chromosome 4 and likely belong to the same gene family. Our findings provide novel insights in the evolution of R gene clusters conferring late-blight resistance in Solanum spp.