A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24

• Published in: Journal of virological methods Vol. 157; no. 1; pp. 98 - 101
• Main Authors: Biancotto, Angélique, Brichacek, Beda, Chen, Silvia S., Fitzgerald, Wendy, Lisco, Andrea, Vanpouille, Christophe, Margolis, Leonid, Grivel, Jean-Charles
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01-04-2009; Elsevier
• Subjects: Biological and medical sciences; Detection; Diagnosis; Flow Cytometry - methods; Fluorescent Antibody Technique, Direct - methods; Fundamental and applied biological sciences. Psychology; HIV Core Protein p24 - analysis; HIV-1; HIV-1 - chemistry; Human immunodeficiency virus 1; Humans; Microbiology; Microspheres; Multiplex assay; p24; Sensitivity and Specificity; Techniques used in virology; Virology; HIV-1; Multiplex assay; p24; Diagnosis; Detection; Microbiology; HIV-1 virus; Retroviridae; Method; Lentivirus; Virology; Virus; Human immunodeficiency virus; H1V-1
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2008.11.013

Nucleic acid measurements are used to follow HIV-1 viral load in clinical applications while p24 ELISA is commonly used to monitor HIV-1 replication in research settings. Current ELISA assays are expensive and offer a narrow dynamic measurement range. This report describes a simple, sensitive and inexpensive bead-based assay offering a wide dynamic measurement range. This cytometric bead assay allows the detection of p24 concentrations over 4 orders of magnitude from less than 0.4
pg to up to 20,000
pg
ml
−1 in a volume of 50
μl and can be combined with other measurements.