Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México

• Published in: The Canadian journal of infectious diseases & medical microbiology Vol. 2017; no. 2017; pp. 1 - 7
• Main Authors: Ramírez Durán, Ninfa, Moreno Pérez, Pablo, Isaac-Olive, Keila, Valladares Carranza, Benjamín, Rivero Pérez, Nallely, Zaragoza Bastida, Adrian, Sandoval-Trujillo, Ángel H.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2017; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Bacteria; Cattle; Diseases; Endocarditis; Exudates; Exudation; Gene sequencing; Genetic aspects; Genotype; Identification; Identification and classification; Infections; Infectious diseases; Markers; Milk; Molecular biology; Molecular diagnostic techniques; Mycobacteria; Mycobacterium; Public health; rRNA 16S; rRNA 23S; Skin; Species; Tuberculosis; Zoonoses; Mexico
• ISSN: 1712-9532; 1918-1493
• DOI: 10.1155/2017/6094587

Mycobacterium genus causes a variety of zoonotic diseases. The best known example is the zoonotic tuberculosis due to M. bovis. Much less is known about “nontuberculous mycobacteria (NTM),” which are also associated with infections in humans. The Mexican standard NOM-ZOO-031-1995 regulates the presence of M. bovis in cattle; however, no regulation exists for the NTM species. The objective of this study was to isolate and identify nontuberculous mycobacteria species from cattle of local herds in the south region of the State of Mexico through the identification and detection of the 100 bp molecular marker in the 23S rRNA gene with subsequent sequencing of the 16S rRNA gene. Milk samples (35) and nasal exudate samples (68) were collected. From the 108 strains isolated, 39 were selected for identification. Thirteen strains isolated from nasal exudates amplified the 100 bp molecular marker and were identified as M. neoaurum (six strains), M. parafortuitum (four strains), M. moriokaense (two strains), and M. confluentis (one strain). Except M. parafortuitum, the other species identified are of public health and veterinary concern because they are pathogenic to humans, especially those with underlying medical conditions.