Cloning simulation in the cage environment

• Published in: Nucleic acids research Vol. 14; no. 1; pp. 285 - 297
• Main Authors: Douthart, R.J, Thomas, J.J, Rosier, S.D, Schmaltz, J.E, West, J.W
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 10-01-1986
• Subjects: Applied sciences; Base Sequence; Biological and medical sciences; cloning; Cloning, Molecular; computer aided genetic engineering; computer analysis; computer graphics; computer simulation; computer software; computer techniques; computer-aided design; Computers; Deoxyribonuclease BamHI; Diverse techniques; DNA Restriction Enzymes - metabolism; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Genetic Engineering; Hepatitis B Core Antigens - genetics; Hepatitis B virus; Information Systems; Molecular and cellular biology; Other techniques and industries; Plasmids; Software; structural genes; vectors; viral antigens; Technique; Investigation method; Simulation; Molecular cloning; DNA
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/14.1.285

The CAGE/GEM software toolkit for genetic engineering is briefly described. The system functionally uses color graphics and is menu driven. It integrates genetics and features information ("Overlays") with information based on sequence analysis ("Representations"). The system is structured around CAD (Computer Aided Design) principles. The CAGE (Computer Aided Genetic Engineering) aspects of the software are emphasized and illustrated by a simulated cloning of the hepatitis B core antigen gene into the BAMHI site of plasmid pBR322.