Predomination of IL-17-producing tryptase-positive/chymase-positive mast cells in azoospermic chronic testicular inflammation

• Published in: Andrologia Vol. 48; no. 6; pp. 617 - 625
• Main Authors: Chen, S.-J., Duan, Y.-G., Haidl, G., Allam, J.-P.
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01-08-2016; Wiley Subscription Services, Inc
• Subjects: Azoospermia; Azoospermia - metabolism; Azoospermia - pathology; chronic testicular inflammation; Chymases - metabolism; Humans; IL-17; immunohistochemistry; Inflammation - metabolism; Inflammation - pathology; Interleukin-10 - metabolism; Interleukin-17 - metabolism; Interleukin-1beta - metabolism; Male; Mast Cells - metabolism; MC tryptase/chymase; Testis - metabolism; Testis - pathology; Tryptases - metabolism; Tumor Necrosis Factor-alpha - metabolism; Azoospermia; chronic testicular inflammation; immunohistochemistry; IL-17; MC tryptase/chymase
• ISSN: 0303-4569; 1439-0272
• DOI: 10.1111/and.12487

Summary
Chronic testicular inflammation and infection have been regarded as important factors in the pathogenesis of azoospermia. As key effector cells in innate and adaptive immune system, mast cells (MCs) were observed in inflammation and autoimmune disease. Furthermore, increased expression of tryptase‐positive MCs has been reported in testicular disorders associated with male infertility/subfertility. However, little is known about the potential relationship between MCs and chronic testicular inflammation in azoospermic patients. Moreover, the preferential expression of MCs' subtypes in testis of these patients is still far from being understood. Thus, this study aimed to investigate characteristics of testicular MCs as well as their subtypes in azoospermic men with chronic testicular inflammation (AZI, n = 5) by immunohistochemical techniques. Our results showed significant increase of MCs in AZI, and more importantly, considerable numbers of tryptase‐positive/chymase‐positive MCs could also be demonstrated in AZI, when compared to control groups representing azoospermia without chronic testicular inflammation (AZW, n = 5) and normal spermatogenesis (NT, n = 5) respectively. Most interestingly, immunofluorescence staining revealed autoimmune‐associated interleukin (IL)‐17‐producing MCs in AZI, whereas co‐expression of MC markers with tumour necrosis factor (TNF)‐α, IL‐10 and IL‐1β could not be detected. In conclusion, AZI is associated with significant increase of tryptase‐positive/chymase‐positive MCs expressing IL‐17, and these MCs might contribute to the pathogenesis of AZI.