Distinct expression and function of three ammonium transporter genes (OsAMT1;1-1;3) in rice [Oryza sativa]

To study the regulation of ammonium uptake into rice roots, three ammonium transporter genes (OsAMT1;1, 1;2 and 1;3; Oryza sativa ammonium transporter) were isolated and examined. OsAMT1s belong to AMT1 family, containing 11 putative transmembrane-spanning domains. Southern blot analysis and screeni...

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Published in:Plant and cell physiology Vol. 44; no. 7; pp. 726 - 734
Main Authors: Sonoda, Y. (Hokkaido Univ., Sapporo (Japan)), Ikeda, A, Saiki, S, von Wiren, N.v, Yamaya, T, Yamaguchi, J
Format: Journal Article
Language:English
Published: Japan Oxford University Press 01-07-2003
Oxford Publishing Limited (England)
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Summary:To study the regulation of ammonium uptake into rice roots, three ammonium transporter genes (OsAMT1;1, 1;2 and 1;3; Oryza sativa ammonium transporter) were isolated and examined. OsAMT1s belong to AMT1 family, containing 11 putative transmembrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1-1;3 the complete AMT1 family of rice had been isolated. Heterologous expression of OsAMT1s in the yeast Saccharomyces cerevisiae mutant 31019b showed that all three OsAMT1s exhibit ammonium transport activity. Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3. In situ mRNA detection revealed that OsAMT1; 2 is expressed in the central cylinder and cell surface of root tips. This gene expression analysis revealed a distinct nitrogen-dependent regulation for AMTs in rice, differing from that in tomato or Arabidopsis.
Bibliography:2004004033
F61
F30
ark:/67375/HXZ-TLFC4FCZ-F
Received February 27, 2003; Accepted April 21, 2003
istex:C765FB2DF58A485904E24BFFAF8077965EBB754D
local:pcg083
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ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcg083