Localization of the glnD gene on a revised map of the 200-kilobase region of the Escherichia coli chromosome

To identify the physical location of glnD, we made use of a glnD::Tn10 insertion in strain RB9040 (3). Southern blotting of PstI-, EcoRI-, and EcoRI-SalI-digested genomic DNAs of RB9040 and its wild-type parent RB9010, using 21C8 phage DNA as a probe. This experiment located the Tn10 in strain RB904...

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Bibliographic Details
Published in:	Journal of bacteriology Vol. 174; no. 5; pp. 1702 - 1703
Main Authors:	VAN HEESWIJK, W, KUPPINGER, O, MERRICK, M, KAHN, D
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-03-1992
Subjects:	Bacteriology Biological and medical sciences Chromosome Mapping Deoxyribonucleases, Type II Site-Specific - metabolism DNA, Bacterial - metabolism Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial - genetics Genetics Life Sciences Microbiology Other Restriction Mapping UDPglucose-Hexose-1-Phosphate Uridylyltransferase - genetics Genetic mapping Enzyme Escherichia coli Restriction map Bacteria Chromosome Glutamine synthetase Localization Enterobacteriaceae
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