Cloacibacterium normanense gen. nov., sp. nov., a novel bacterium in the family Flavobacteriaceae isolated from municipal wastewater

• Published in: International journal of systematic and evolutionary microbiology Vol. 56; no. 6; pp. 1311 - 1316
• Main Authors: Allen, Toby D, Lawson, Paul A, Collins, Matthew D, Falsen, Enevold, Tanner, Ralph S
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-06-2006; Society for General Microbiology
• Subjects: acid; activated-sludge; Bacteriology; Base Composition; Biological and medical sciences; classification; Cloacibacterium normanense; cytophagas; DNA, Bacterial - chemistry; emended description; Flavobacteriaceae; Flavobacteriaceae - classification; Flavobacteriaceae - genetics; Flavobacteriaceae - isolation & purification; Fundamental and applied biological sciences. Psychology; Microbiology; Microbiology in the medical area; Mikrobiologi inom det medicinska området; Miscellaneous; Molecular Sequence Data; Phylogeny; plant; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; soil; Sweden; Systematics; Waste Disposal, Fluid; Water Microbiology; Sweden; Bacteria; New genus; Flavobacteriaceae; Taxonomy; Waste water
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.64218-0

1 Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA
2 School of Food Biosciences, University of Reading, Reading RG6 6AP, UK
3 Culture Collection, Department of Clinical Bacteriology, University of Göteborg, SE-413 46 Göteborg, Sweden
Correspondence Ralph S. Tanner rtanner{at}ou.edu
Phenotypic and phylogenetic studies were performed on three isolates of an unknown Gram-negative, facultatively anaerobic, non-motile, yellow-pigmented, rod-shaped organism isolated from raw sewage. 16S rRNA gene sequence analysis indicated that these strains were members of the Bergeyella–Chryseobacterium–Riemerella branch of the family Flavobacteriaceae . The unknown bacterium was readily distinguished from reference strains by 16S rRNA gene sequencing and biochemical tests. The organism contained menaquinone MK-6 as the predominant respiratory quinone and had a DNA G+C content of 31 mol%. A most probable number-PCR approach was developed to detect, and estimate the numbers of, this organism. Untreated wastewater from one plant yielded an estimated count of 1.4 x 10 5  cells ml –1 , and untreated wastewater from a second plant yielded an estimated count of 1.4 x 10 4  cells ml –1 . Signal was not detected from treated effluent or from human stool specimens. On the basis of the results of the study presented, it is proposed that the unknown bacterium be classified in a novel genus Cloacibacterium , as Cloacibacterium normanense gen. nov., sp. nov., which is also the type species. The type strain of Cloacibacterium normanense is strain NRS1 T (=CCUG 46293 T =CIP 108613 T =ATCC BAA-825 T =DSM 15886 T ).
Abbreviations: MPN, most probable number
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CCUG 46293 T is AJ575430 .