Dietary Effect on the Proteome of the Common Octopus ( Octopus vulgaris ) Paralarvae

Dietary Effect on the Proteome of the Common Octopus ( Octopus vulgaris ) Paralarvae

Nowadays, the common octopus ( ) culture is hampered by massive mortalities occurring during early life-cycle stages (paralarvae). Despite the causes of the high paralarvae mortality are not yet well-defined and understood, the nutritional stress caused by inadequate diets is pointed out as one of t...

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Published in:Frontiers in physiology Vol. 8; p. 309
Main Authors: Varó, Inmaculada, Cardenete, Gabriel, Hontoria, Francisco, Monroig, Óscar, Iglesias, José, Otero, Juan J, Almansa, Eduardo, Navarro, Juan C
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 17-05-2017
Subjects:
novel biomarkers
nutritional stress
Octopus vulgaris
paralarvae
Physiology
proteome
welfare
Octopus vulgaris
novel biomarkers
paralarvae
welfare
nutritional stress
proteome
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Summary:Nowadays, the common octopus ( ) culture is hampered by massive mortalities occurring during early life-cycle stages (paralarvae). Despite the causes of the high paralarvae mortality are not yet well-defined and understood, the nutritional stress caused by inadequate diets is pointed out as one of the main factors. In this study, the effects of diet on paralarvae is analyzed through a proteomic approach, to search for novel biomarkers of nutritional stress. A total of 43 proteins showing differential expression in the different conditions studied have been identified. The analysis highlights proteins related with the carbohydrate metabolism: glyceraldehyde-3-phosphate-dedydrogenase (GAPDH), triosephosphate isomerase; other ways of energetic metabolism: NADP -specific isocitrate dehydrogenase, arginine kinase; detoxification: glutathione-S-transferase (GST); stress: heat shock proteins (HSP70); structural constituent of eye lens: S-crystallin 3; and cytoskeleton: actin, actin-beta/gamma1, beta actin. These results allow defining characteristic proteomes of paralarvae depending on the diet; as well as the use of several of these proteins as novel biomarkers to evaluate their welfare linked to nutritional stress. Notably, the changes of proteins like S-crystallin 3, arginine kinase and NAD specific isocitrate dehydrogenase, may be related to fed vs. starving paralarvae, particularly in the first 4 days of development.
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This article was submitted to Invertebrate Physiology, a section of the journal Frontiers in Physiology
Edited by: Fernando Ariel Genta, Oswaldo Cruz Foundation, Brazil
Reviewed by: Germán Leandro Rosano, Instituto de Biología Molecular y Celular de Rosario, Argentina; Jesper Givskov Sørensen, Aarhus University, Denmark
ISSN:1664-042X
1664-042X
DOI:10.3389/fphys.2017.00309