Spectrophotometric method for the determination of chromium (VI) in water samples

• Published in: Environmental monitoring and assessment Vol. 157; no. 1-4; pp. 575 - 582
• Main Authors: Nagaraj, P., Aradhana, N., Shivakumar, Anantharaman, Shrestha, Ashwinee Kumar, k Gowda, Avinash
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-10-2009; Springer; Springer Nature B.V
• Subjects: Animal, plant and microbial ecology; Applied ecology; Applied sciences; Atmospheric Protection/Air Quality Control/Air Pollution; Azo Compounds - analysis; Azo Compounds - chemistry; Biological and medical sciences; Chromium; Chromium - analysis; Dapsone - analysis; Dapsone - chemistry; Earth and Environmental Science; Ecology; Ecotoxicology; Ecotoxicology, biological effects of pollution; Environment; Environmental Management; Environmental monitoring; Environmental Monitoring - methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Hydroxylamine - analysis; Hydroxylamine - chemistry; Monitoring/Environmental Analysis; Naphthalenes - analysis; Naphthalenes - chemistry; Oxidation; Pollution; Reagents; Sampling techniques; Spectrophotometry - methods; Water - chemistry; Water analysis; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - chemistry; Water quality; Water sampling; Dapsone; Chromium (VI) determination; N-(1-Napthyl) Ethylenediamine dihydrochloride; Spectrophotometry; Method; Chromium VI
• ISSN: 0167-6369; 1573-2959
• DOI: 10.1007/s10661-008-0557-2

A simple and sensitive spectrophotometric method for the determination of chromium has been developed. The method is based on the diazotization of Dapsone in hydroxylamine hydrochloride medium and coupling with N-(1-Napthyl) Ethylene Diamine Dihydrochloride by electrophilic substitution to produce an intense pink azo-dye, which has absorption maximum at 540 nm. The Beer’s law is obeyed from 0.02-1.0 μg mL
−1
and the molar absorptivity is 3.4854 L mol
−1
cm
−1
. The Limits of quantification and Limit of detection of the proposed method are 0.0012 μg mL
−1
and 0.0039 μg mL
−1
respectively. The method has been successfully applied for the determination of chromium in water samples and the results were statistically evaluated with that of the reference method.