Specificity of antibodies: Unexpected cross-reactivity of antibodies directed against the excitatory amino acid transporter 3 (EAAT3)

Specific antibodies are essential tools for identifying individual proteins in biological samples. While generation of antibodies is often straightforward, determination of the antibody specificity is not. Here we illustrate this by describing the production and characterization of antibodies to exc...

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Bibliographic Details
Published in:	Neuroscience Vol. 136; no. 3; pp. 649 - 660
Main Authors:	Holmseth, S., Dehnes, Y., Bjørnsen, L.P., Boulland, J.-L., Furness, D.N., Bergles, D., Danbolt, N.C.
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 2005 Elsevier
Subjects:	Amino Acid Sequence Animals antibodies Antibodies - metabolism Antibody Affinity - physiology Antibody Specificity - physiology Antigen-Antibody Reactions Biological and medical sciences Blotting, Western - methods Brain - metabolism Brain - ultrastructure Enzyme-Linked Immunosorbent Assay - methods Epitopes - immunology Epitopes - metabolism Excitatory Amino Acid Transporter 3 - immunology Excitatory Amino Acid Transporter 3 - metabolism Fundamental and applied biological sciences. Psychology glutamate uptake Immunization - methods immunocytochemistry Immunohistochemistry - methods Mice Microscopy, Immunoelectron - methods Myelin Basic Protein - metabolism oligodendrocyte Peptides - immunology Peptides - metabolism polyreactive Rabbits Rats Sensitivity and Specificity Sheep specificity testing tubulin Tubulin - metabolism Vertebrates: nervous system and sense organs SDS polyreactive KLH oligodendrocyte glutamate uptake tubulin NaPi TBST immunocytochemistry EDTA specificity testing CHAPS EAAT BSA SDS-PAGE PMSF HEPES HSA EAAC1 antibodies MBP Map MBS NSC Immunocytochemistry Neuroglia Glutamate Uptake Tubulin Oligodendrocyte Cross reaction Neurotransmitter Excitatory aminoacid polyre- active Carrier protein
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