Modification of wheat straw lignin by solid state fermentation with white-rot fungi

• Published in: Bioresource technology Vol. 100; no. 20; pp. 4829 - 4835
• Main Authors: Dinis, Maria J., Bezerra, Rui M.F., Nunes, Fernando, Dias, Albino A., Guedes, Cristina V., Ferreira, Luís M.M., Cone, John W., Marques, Guilhermina S.M., Barros, Ana R.N., Rodrigues, Miguel A.M.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-10-2009; Elsevier
• Subjects: Basidiomycota - enzymology; Basidiomycota - metabolism; Biodegradation; Biological and medical sciences; Biotechnology; cellulose; Coumaric Acids - metabolism; degradation; enzymes; Esterases - metabolism; Esterification; Fermentation; feruloyl esterases; Food industries; Fundamental and applied biological sciences. Psychology; Hydrolysis; laccase; Lignin - metabolism; manganese peroxidase; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; phenolic-compounds; Phenols - analysis; plant-cell wall; release; Time Factors; Triticum - metabolism; Use and upgrading of agricultural and food by-products. Biotechnology; Wheat straw; White-rot fungi; Biodegradation; White-rot fungi; Wheat straw; Lignin; Wood destroying fungi; Cereal by product; Solid substrate fermentation
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2009.04.036

The potential of crude enzyme extracts, obtained from solid state cultivation of four white-rot fungi (
Trametes versicolor,
Bjerkandera adusta,
Ganoderma applanatum and
Phlebia rufa), was exploited to modify wheat straw cell wall. At different fermentation times, manganese-dependent peroxidase (MnP), lignin peroxidase (LiP), laccase, carboxymethylcellulase (CMCase), avicelase, xylanase and feruloyl esterase activities were screened and the content of lignin as well as hydroxycinnamic acids in fermented straw were determined. All fungi secreted feruloyl esterase while LiP was only detected in crude extracts from
B. adusta. Since no significant differences (
P
>
0.05) were observed in remaining lignin content of fermented straw, LiP activity was not a limiting factor of enzymatic lignin removal process. The levels of esterified hydroxycinnamic acids degradation were considerably higher than previous reports with lignocellulosic biomass. The data show that
P. rufa, may be considered for more specific studies as higher ferulic and
p-coumaric acids degradation was observed for earlier incubation times.