The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis

• Published in: Journal of investigative dermatology Vol. 135; no. 8; pp. 2109 - 2118
• Main Authors: Herro, Rana, Antunes, Ricardo Da S., Aguilera, Amelia R., Tamada, Koji, Croft, Michael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-08-2015; Elsevier Limited
• Subjects: Animals; Bleomycin - pharmacology; Cells, Cultured; Collagen - metabolism; Disease Models, Animal; Female; Fibrosis - metabolism; Fibrosis - pathology; Fibrosis - physiopathology; Humans; Immunoglobulins - deficiency; Immunoglobulins - genetics; Immunoglobulins - metabolism; Infant, Newborn; Keratinocytes - drug effects; Keratinocytes - physiology; Mice; Mice, Inbred C57BL; Mice, Knockout; Receptors, Cytokine - deficiency; Receptors, Cytokine - genetics; Receptors, Cytokine - metabolism; Receptors, Tumor Necrosis Factor, Member 14 - deficiency; Receptors, Tumor Necrosis Factor, Member 14 - genetics; Receptors, Tumor Necrosis Factor, Member 14 - metabolism; Skin - metabolism; Skin - pathology; Skin - physiopathology; Tumor Necrosis Factor Ligand Superfamily Member 14 - deficiency; Tumor Necrosis Factor Ligand Superfamily Member 14 - genetics; Tumor Necrosis Factor Ligand Superfamily Member 14 - physiology
• ISSN: 0022-202X; 1523-1747
• DOI: 10.1038/jid.2015.110

Several inflammatory diseases including scleroderma and atopic dermatitis display dermal thickening, epidermal hypertrophy, or excessive accumulation of collagen. Factors that might promote these features are of interest for clinical therapy. We previously reported that LIGHT, a TNF superfamily molecule, mediated collagen deposition in the lungs in response to allergen. We therefore tested whether LIGHT might similarly promote collagen accumulation and features of skin fibrosis. Strikingly, injection of recombinant soluble LIGHT into naive mice, either subcutaneously or systemically, promoted collagen deposition in the skin and dermal and epidermal thickening. This replicated the activity of bleomycin, an antibiotic that has been previously used in models of scleroderma in mice. Moreover skin fibrosis induced by bleomycin was dependent on endogenous LIGHT activity. The action of LIGHT in vivo was mediated via both of its receptors, HVEM and LTβR, and was dependent on the innate cytokine TSLP and TGF-β. Furthermore, we found that HVEM and LTβR were expressed on human epidermal keratinocytes and that LIGHT could directly promote TSLP expression in these cells. We reveal an unappreciated activity of LIGHT on keratinocytes and suggest that LIGHT may be an important mediator of skin inflammation and fibrosis in diseases such as scleroderma or atopic dermatitis.