Detecting episomal or integrated human papillomavirus 16 DNA using an exonuclease V-qPCR-based assay

Screening for human papillomavirus (HPV) integration into host cell chromosomes typically requires large amounts of time and reagents. We developed a rapid and sensitive assay based on exonuclease V (ExoV) and quantitative polymerase chain reaction (qPCR) to determine HPV genome configurations in ce...

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Bibliographic Details
Published in:	Virology (New York, N.Y.) Vol. 537; pp. 149 - 156
Main Authors:	Myers, J.E., Guidry, J.T., Scott, M.L., Zwolinska, K., Raikhy, G., Prasai, K., Bienkowska-Haba, M., Bodily, J.M., Sapp, M.J., Scott, R.S.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-11-2019
Subjects:	Cells, Cultured DNA, Viral - analysis DNA, Viral - genetics Episomal Exodeoxyribonuclease V - metabolism Exonuclease V HPV16 Human papillomavirus 16 - genetics Human papillomavirus 16 - growth & development Humans Integrated Plasmids Proviruses - genetics qPCR Real-Time Polymerase Chain Reaction - methods Virus Integration Exonuclease V qPCR Integrated Episomal HPV16
Online Access:	Get full text
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