Comprehensive analysis of phenolics compounds in citrus fruits peels by UPLC-PDA and UPLC-Q/TOF MS using a fused-core column

• Published in: Food Chemistry: X Vol. 14; p. 100262
• Main Authors: Sanches, Vitor L., Cunha, Tanize A., Viganó, Juliane, de Souza Mesquita, Leonardo M., Faccioli, Lúcia Helena, Breitkreitz, Marcia Cristina, Rostagno, Maurício A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 30-06-2022; Elsevier
• Subjects: Analytical Methods; Citrus peels; Comprehensive analysis; Fused-core column; Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry; Ultra-performance liquid chromatography coupled to photodiode array detection; Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry; Ultra-performance liquid chromatography coupled to photodiode array detection; Comprehensive analysis; Fused-core column; Citrus peels
• ISSN: 2590-1575; 2590-1575
• DOI: 10.1016/j.fochx.2022.100262

•A comprehensive UPLC-PDA/UPLC-HRMS method was developed for citrus peels.•In this study, 24 phenolic compounds and 5 limonoids in lime, lemon, and rangpur lime samples were identified.•The method is short compared to others recently published.
In this work, a method based on ultra-high-performance liquid chromatography with a photodiode array detector (UPLC-PDA) was developed to comprehensively analyze phenolic compounds in peels of lime (Citrus × latifolia), lemon (Citrus limon), and rangpur lime (Citrus × limonia). The reverse-phase separation was achieved with a C18 fused-core column packed with the smallest particles commercially available (1.3 um). The method was successfully coupled with high-resolution mass spectrometry (HRMS), allowing the detection of 24 phenolic compounds and five limonoids in several other citrus peels species: key lime, orange and sweet orange, tangerine, and tangerine ponkan, proving the suitability for comprehensive analysis in citrus peel matrices. Additionally, the developed method was validated according to the Food and drug administration (FDA) and National Institute of Metrology Quality and Technology (INMETRO) criteria, demonstrating specificity, linearity, accuracy, and precision according to these guidelines. System suitability parameters such as resolution, tailoring, plate count were also verified.