Linking N2O emissions from biochar-amended soil to the structure and function of the N-cycling microbial community

• Published in: The ISME Journal Vol. 8; no. 3; pp. 660 - 674
• Main Authors: Harter, Johannes, Krause, Hans-Martin, Schuettler, Stefanie, Ruser, Reiner, Fromme, Markus, Scholten, Thomas, Kappler, Andreas, Behrens, Sebastian
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01-03-2014; Nature Publishing Group
• Subjects: 631/326/171/1818; 631/326/2565/855; 631/326/47; Anthropogenic factors; Biomedical and Life Sciences; Charcoal; Charcoal - chemistry; Charcoal - metabolism; Community structure; Crop yield; Denitrification; Ecology; Emissions; Evolutionary Biology; Farm buildings; Fertilizer application; Fertilizers; Greenhouse gases; High temperature; Life Sciences; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microorganisms; Nitrification; Nitrogen - chemistry; Nitrogen cycle; Nitrogen fixation; Nitrous oxide; Nitrous Oxide - metabolism; Original; original-article; Pyrolysis; Saturated soils; Soil - chemistry; Soil amendment; Soil Microbiology; Soil quality; soil microbial community; biochar; O emission; nitrous oxide; nitrification; nitrogen cycle; denitrification; N; greenhouse gas
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/ismej.2013.160

Nitrous oxide (N
2
O) contributes 8% to global greenhouse gas emissions. Agricultural sources represent about 60% of anthropogenic N
2
O emissions. Most agricultural N
2
O emissions are due to increased fertilizer application. A considerable fraction of nitrogen fertilizers are converted to N
2
O by microbiological processes (that is, nitrification and denitrification). Soil amended with biochar (charcoal created by pyrolysis of biomass) has been demonstrated to increase crop yield, improve soil quality and affect greenhouse gas emissions, for example, reduce N
2
O emissions. Despite several studies on variations in the general microbial community structure due to soil biochar amendment, hitherto the specific role of the nitrogen cycling microbial community in mitigating soil N
2
O emissions has not been subject of systematic investigation. We performed a microcosm study with a water-saturated soil amended with different amounts (0%, 2% and 10% (w/w)) of high-temperature biochar. By quantifying the abundance and activity of functional marker genes of microbial nitrogen fixation (
nifH
), nitrification (
amoA
) and denitrification (
nirK
,
nirS
and
nosZ
) using quantitative PCR we found that biochar addition enhanced microbial nitrous oxide reduction and increased the abundance of microorganisms capable of N
2
-fixation. Soil biochar amendment increased the relative gene and transcript copy numbers of the
nosZ
-encoded bacterial N
2
O reductase, suggesting a mechanistic link to the observed reduction in N
2
O emissions. Our findings contribute to a better understanding of the impact of biochar on the nitrogen cycling microbial community and the consequences of soil biochar amendment for microbial nitrogen transformation processes and N
2
O emissions from soil.