Characterization of mixed disomic and polysomic inheritance in the octoploid strawberry (Fragaria x ananassa) using AFLP mapping

• Published in: Theoretical and applied genetics Vol. 107; no. 4; pp. 619 - 628
• Main Authors: LERCETEAU-KÖHLER, E, GUERIN, G, LAIGRET, F, DENOYES-ROTHAN, B
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-08-2003; Berlin Springer Nature B.V; Springer Verlag
• Subjects: Agronomy. Soil science and plant productions; Base Sequence; Biological and medical sciences; Chromosome Mapping; Classical and quantitative genetics. Population genetics. Molecular genetics; Classical genetics, quantitative genetics, hybrids; Crosses, Genetic; Diploidy; DNA, Plant - genetics; Fragaria - genetics; Fundamental and applied biological sciences. Psychology; Gene Dosage; Generalities. Genetics. Plant material; Genetics; Genetics and breeding of economic plants; Genetics of eukaryotes. Biological and molecular evolution; Genome, Plant; Genomics; Life Sciences; Molecular genetics; Polymorphism, Genetic; Polyploidy; Pteridophyta, spermatophyta; Segregation; Vegetals; Chromosomal aberration; Genetic marker; Small fruits; Rosaceae; Fragaria ananassa; Aneuploidy; Linkage map; Fragaria × ananassa; Genetic determinism; Characterization; Dicotyledones; Linkage group; Strawberry; Angiospermae; Octoploidy; AFLPs; Genetic mapping; Polyploidy; Single dose; Y-Disomy; Restriction fragment; Meiosis; Spermatophyta; Inheritance(genetics); Genome; Amplified fragment length polymorphism marker; FRAISIER; AMELIORATION DES PLANTES; GENETIQUE; AFLPS; strawberry; polyploidy; Fragaria x ananassa; linkage map
• ISSN: 0040-5752; 1432-2242; 1432-2242
• DOI: 10.1007/s00122-003-1300-6

A two-way pseudo-testcross strategy, combined with Single Dose Restriction Fragment (SDRF) marker analysis, was used for genetic mapping in the octoploid cultivated strawberry Fragaria x ananassa (2n = 8 x = 56). Based on a 113 full-sib progeny from a cross between the variety Capitola and the clone CF1116, we generated two parental maps using Amplified Fragment Length Polymorphism (AFLP) markers. Ninety two percent of the markers (727 out of 789) showed ratios corresponding to simplex markers (the majority being SDRF markers), and 8% (62 out of 789) fitted a multiplex ratio. Linkage maps were first established using SDRF markers in coupling phase. The female map comprised 235 markers distributed among 43 co-segregation groups, giving a map size of 1,604 cM. On the male map, 280 markers were assigned to 43 co-segregation groups, yielding a map size of 1,496 cM. Once the co-segregation groups were established, their association was tested using repulsion-phase markers. In total, taking into account associations representing the same linkage groups, 30 linkage groups were detected on the female side and 28 on the male side. On the female map, 68.3% of the pairwise marker linkages were in coupling versus 31.7% in repulsion phase, and the corresponding figures on the male map were 72.2% and 27.8%, respectively. In addition, both groups linked only in the coupling phase and groups linked in the repulsion phase were characterized. The observations suggest that the meiotic behavior of the F. x ananassa genome is neither fully disomic nor fully polysomic, but rather mixed. The genome may not be as completely diploidized as previously assumed.