Lambda exonuclease digestion of CGG trinucleotide repeats

• Published in: European biophysics journal Vol. 39; no. 2; pp. 337 - 343
• Main Authors: Conroy, R. S, Koretsky, A. P, Moreland, J
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-01-2010; Springer-Verlag; Springer Nature B.V
• Subjects: Absorption; Bacteriophage phi X 174; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biophysics Letter; Cell Biology; Deoxyribonucleic acid; DNA; Exonucleases - chemistry; Fluorescence; Fragile X Mental Retardation Protein - chemistry; Fragile X Mental Retardation Protein - genetics; Fragile X syndrome; Genetic disorders; Genomics; Lambda exonuclease; Life Sciences; Magnetic tweezers; Membrane Biology; Nanotechnology; Neurobiology; Single molecule; Spectrometry, Fluorescence; Temperature; Time Factors; Trinucleotide Repeats; Magnetic tweezers; Lambda exonuclease; Biophysics; Fragile X syndrome; Single molecule
• ISSN: 0175-7571; 1432-1017; 1432-1017
• DOI: 10.1007/s00249-009-0502-5

Fragile X syndrome and other trinucleotide diseases are characterized by an elongation of a repeating DNA triplet. The ensemble-averaged lambda exonuclease digestion rate of different substrates, including one with an elongated FMR1 gene containing 120 CGG repeats, was measured using absorption and fluorescence spectroscopy. By use of magnetic tweezers sequence-dependent digestion rates and pausing was measured for individual lambda exonucleases. Within the triplet repeats a lower average and narrower distribution of rates and a higher frequency of pausing was observed.