Depletion of Blautia Species in the Microbiota of Obese Children Relates to Intestinal Inflammation and Metabolic Phenotype Worsening

• Published in: mSystems Vol. 5; no. 2
• Main Authors: Benítez-Páez, Alfonso, Gómez Del Pugar, Eva M, López-Almela, Inmaculada, Moya-Pérez, Ángela, Codoñer-Franch, Pilar, Sanz, Yolanda
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 24-03-2020
• Subjects: Body mass index; Chemokines; Children; Children & youth; Cholesterol; Cross-sectional studies; Cytokines; Diabetes mellitus (non-insulin dependent); Digestive system; Gastrointestinal tract; Genotype & phenotype; Girls; Homeostasis; Host-Microbe Biology; Inflammation; Insulin; Insulin resistance; Intestinal microflora; Intestine; Lipoproteins; Metabolism; Metabolites; Microbiota; Monocyte chemoattractant protein; Monocyte chemoattractant protein 1; Monocytes; Obesity; Peripheral blood; Phenotypes; Risk factors; rRNA 16S; Species; Studies; Tumor necrosis factor-TNF; Tumor necrosis factor-α; γ-Interferon; microbiota; Blautia luti; Blautia wexlerae; PBMCs; gut microbiota; permubiome; children; inflammation; childhood obesity; gut inflammation; insulin resistance; obesity; probiotics
• ISSN: 2379-5077; 2379-5077
• DOI: 10.1128/mSystems.00857-19

Cross-sectional studies conducted with obese and control subjects have suggested associations between gut microbiota alterations and obesity, but the links with specific disease phenotypes and proofs of causality are still scarce. The present study aimed to profile the gut microbiota of lean and obese children with and without insulin resistance to characterize associations with specific obesity-related complications and understand the role played in metabolic inflammation. Through massive sequencing of 16S rRNA gene amplicons and data analysis using a novel permutation approach, we have detected decreased incidence of
species, especially
and
, in the gut microbiota of obese children, which was even more pronounced in cases with both obesity and insulin resistance. There was also a parallel increase in proinflammatory cytokines and chemokines (gamma interferon [IFN-γ], tumor necrosis factor alpha [TNF-α], and monocyte chemoattractant protein 1 [MCP-1]) in feces of obese children compared to those of lean ones.
and
were also shown to exert an anti-inflammatory effect in peripheral blood mononuclear cell cultures
, compared to non-obesity-associated species. We suggest that the depletion of
and
species in the gut ecosystem may occur in cases of obesity and contribute to metabolic inflammation leading to insulin resistance.
Child obesity constitutes a risk factor for developing insulin resistance which, if sustained, could lead to more severe conditions like type 2 diabetes (T2D) in adulthood. Our study identified previously unknown species whose depletion (
and
) is associated with insulin resistance in obese individuals. Our results also indicate that these bacterial species might help to reduce inflammation causally linked to obesity-related complications. Childhood is considered a window of opportunity to tackle obesity. These new findings provide, therefore, valuable information for the future design of microbiota-based strategies for the early prevention of obesity-related complications.