Cloning of Salmonella enterica Serovar Enteritidis Fimbrial Protein SefA as a Surface Protein in Escherichia coli Confers the Ability To Attach to Eukaryotic Cell Lines

• Published in: Applied and Environmental Microbiology Vol. 75; no. 20; pp. 6622 - 6625
• Main Authors: Rank, Douglas L, Saeed, Mahdi A, Muriana, Peter M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-10-2009; American Society for Microbiology (ASM)
• Subjects: Animals; Bacterial Adhesion - genetics; Bacterial Adhesion - physiology; Biological and medical sciences; Cell Line; Cell Membrane - physiology; Chickens; Cloning; Cloning, Molecular; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - physiology; Eukaryotes; Expression vectors; Female; Fimbriae Proteins - genetics; Fimbriae Proteins - physiology; Food Microbiology; Fundamental and applied biological sciences. Psychology; Gene expression; Genes, Bacterial; Genetics and Molecular Biology; Granulosa Cells - microbiology; Humans; Microbiology; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Salmonella; Salmonella enteritidis - genetics; Salmonella enteritidis - pathogenicity; Salmonella enteritidis - physiology; Salmonella Food Poisoning - etiology; Salmonella enteritidis; Cell line; Escherichia coli; Eukaryote; Bacteria; Protein; Enterobacteriaceae
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.00639-09

The gene for the Salmonella enterica serovar Enteritidis fimbrial protein SefA was cloned into an Escherichia coli surface expression vector and confirmed by Western blot assay. E. coli clones expressing SefA attached to avian ovary granulosa cells and HEp-2 cells, providing evidence for the involvement of SefA in the ability of Salmonella to attach to eukaryotic cells.