An impedimetric biosensor for detection of dengue serotype at picomolar concentration based on gold nanoparticles-polyaniline hybrid composites

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 86; no. 2; pp. 414 - 419
• Main Authors: Nascimento, Helena P.O., Oliveira, Maria D.L., de Melo, Celso P., Silva, Gilcelia J.L., Cordeiro, Marli T., Andrade, Cesar A.S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-09-2011
• Subjects: Adsorption; Aniline Compounds - chemistry; Base Pairing; Biosensing Techniques - methods; Biosensors; colloids; Cyclic voltammetry; Dengue; Dengue - diagnosis; Dengue - virology; Dengue Virus - chemistry; Dengue Virus - classification; Dengue Virus - isolation & purification; Dengue Virus - metabolism; Dielectric Spectroscopy; DNA; DNA Primers - chemistry; DNA Primers - metabolism; DNA, Viral - chemistry; DNA, Viral - metabolism; Electrochemical impedance spectroscopy; Electrochemical Techniques - methods; Electrodes; Gold; Gold - chemistry; Gold - metabolism; Humans; Hybrid composites; Limit of Detection; Nanocomposites; Nanomaterials; Nanoparticles - chemistry; Nanostructure; patients; Potentiometry; Sensitivity and Specificity; serotypes; Serotyping - methods; Cyclic voltammetry; Dengue; DNA; Electrochemical impedance spectroscopy
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2011.04.028

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► A novel gold nanoparticles-polyaniline hybrid composite (AuNpPANI) with SH-terminal groups was obtained. ► The AuNpPANI-ST(1–3) systems were able to recognize the dengue serotype of different patients at picomolar concentrations. ► The AuNpPANI-ST system exhibited a highly selectivity response to human patient's dengue genome complementary target.
In this work, we describe the preparation and characterization of a novel gold nanoparticles-polyaniline hybrid composite (AuNpPANI) with SH-terminal groups that, due to its ability of immobilizing dengue serotype-specific primers 1, 2 and 3 (ST1, ST2 and ST3), can be used for the development of biosensors. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were performed. CV and EIS results demonstrated that the AuNpPANI can immobilize ST1, ST2 and ST3, forming AuNpPANI-ST complexes. Well-defined cyclic voltammograms characteristic of a diffusion-limited redox process were observed both for the bare gold electrode and after these electrodes have been modified by the adsorption of AuNpPANI or AuNpPANI-ST. The AuNpPANI-ST(1–3) systems were able to recognize the dengue serotype of different patients at picomolar concentrations. Even when small volumes and low concentrations of the analyte were used, the CV and EIS results showed unequivocal evidence of an existing interaction between dengue serotype-specific primers and their complementary genomic DNA targets.