A single amino acid change in the cytoplasmic domain of the simian immunodeficiency virus transmembrane molecule increases envelope glycoprotein expression on infected cells
Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from...
Saved in:
| Published in: | Journal of Virology Vol. 69; no. 9; pp. 5217 - 5227 |
|---|---|
| Main Authors: | , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
American Society for Microbiology
01-09-1995
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Article Usage Stats
Services
JVI
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley
Reddit
StumbleUpon
Twitter
current issue
Spotlights in the Current Issue
JVI
About
JVI
Subscribers
Authors
Reviewers
Advertisers
Inquiries from the Press
Permissions & Commercial Reprints
ASM Journals Public Access Policy
JVI
RSS Feeds
1752 N Street N.W. • Washington DC 20036
202.737.3600 • 202.942.9355 fax • journals@asmusa.org
Print ISSN:
0022-538X
Online ISSN:
1098-5514
Copyright © 2014
by the
American Society for Microbiology.
For an alternate route to
JVI
.asm.org, visit:
JVI
|
|---|---|
| AbstractList | We have described a virus termed CP-MAC, derived from the BK28 molecular clone of simian immunodeficiency virus, that was remarkable for its ability to infect Sup-T1 cells with rapid kinetics, cell fusion, and CD4 down-modulation (C. C. LaBranche, M. M. Sauter, B. S. Haggarty, P. J. Vance, J. Romano, T. K. Hart, P. J. Bugelski, and J. A. Hoxie, J. Virol. 68:5509-5522, 1994 [Erratum 68:7665-7667]). Compared with BK28, CP-MAC exhibited a number of changes in its envelope glycoproteins, including a highly stable association between the external (SU) and transmembrane (TM) molecules, a more rapid electrophoretic mobility of TM, and, of particular interest, a marked increase in the level of envelope protein expression on the surface of infected cells. These changes were shown to be associated with 11 coding mutations in the env gene (5 in SU and 6 in TM). In this report, we demonstrate that a single amino acid mutation of a Tyr to a Cys at position 723 (Y723C) in the TM cytoplasmic domain of CP-MAC is the principal determinant for the increased expression of envelope glycoproteins on the cell surface. When introduced into the env gene of BK28, the Y723C mutation produced up to a 25-fold increase in the levels of SU and TM on chronically infected cells, as determined by fluorescence-activated cell sorter analysis with monoclonal and polyclonal antibodies. A similar effect was observed when a Tyr-to-Cys change was introduced at the analogous position (amino acid 721) in the SIVmac239 molecular clone, which, unlike BK28 does not contain a premature stop codon in its TM cytoplasmic tail. Substituting other amino acids, including Ala, Ile, and Ser, at this position produced increases in surface envelope glycoproteins that were similar to that observed for the Cys substitution, while a Tyr-to-Phe mutation produced a smaller increase. These results could not be accounted for by differences in the kinetics or efficiency of envelope glycoprotein processing or by shedding of SU from infected cells. However, immunoelectron microscopy demonstrated that the Y723C mutation in BK28 produced a striking redistribution of cell surface envelope molecules from localized patches to a diffuse pattern that covered the entire plasma membrane. This finding suggests that mutation of a Tyr residue in the simian immunodeficiency virus TM cytoplasmic domain may disrupt a structural element that can modulate envelope glycoprotein expression on the surface of infected cells. We have described a virus termed CP-MAC, derived from the BK28 molecular clone of simian immunodeficiency virus, that was remarkable for its ability to infect Sup-T1 cells with rapid kinetics, cell fusion, and CD4 down-modulation. Compared with BK28, CP-MAC exhibited a number of changes in its envelope glycoproteins, including a highly stable association between the external (SU) and transmembrane (TM) molecules, a more rapid electrophoretic mobility of TM, and, of particular interest, a marked increase in the level of envelope protein expression on the surface of infected cells. These changes were shown to be associated with 11 coding mutations in the env gene (5 in SU and 6 in TM). In this report, we demonstrate that a single amino acid mutation of a Tyr to a Cys at position 723 (Y723C) in the TM cytoplasmic domain of CP-MAC is the principal determinant for the increased expression of envelope glycoproteins on the cell surface. When introduced into the env gene of BK28, the Y723C mutation produced up to a 25-fold increase in the levels of SU and TM on chronically infected cells, as determined by fluorescence-activated cell sorter analysis with monoclonal and polyclonal antibodies. A similar effect was observed when a Tyr-to-Cys change was introduced at the analogous position (amino acid 721) in the SIVmac239 molecular clone, which, unlike BK28 does not contain a premature stop codon in its TM cytoplasmic tail. Substituting other amino acids, including Ala, Ile, and Ser, at this position produced increases in surface envelope glycoproteins that were similar to that observed for the Cys substitution, while a Tyr-to-Phe mutation produced a smaller increase. These results could not be accounted for by differences in the kinetics or efficiency of envelope glycoprotein processing or by shedding of SU from infected cells. However, immunoelectron microscopy demonstrated that the Y723C mutation in BK28 produced a striking redistribution of cell surface envelope molecules from localized patches to a diffuse pattern that covered the entire plasma membrane. This finding suggests that mutation of a Tyr residue in the simian immunodeficiency virus TM cytoplasmic domain may disrupt a structural element that can modulate envelope glycoprotein expression on the surface of infected cells. Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JVI .asm.org, visit: JVI |
| Author | J A Hoxie J Romano P J Bugelski B S Haggarty M Marsh P J Vance M M Sauter T K Hart C C LaBranche |
| AuthorAffiliation | Hematology-Oncology Division, Hospital of the University of Pennsylvania, Philadelphia 19104, USA |
| AuthorAffiliation_xml | – name: Hematology-Oncology Division, Hospital of the University of Pennsylvania, Philadelphia 19104, USA |
| Author_xml | – sequence: 1 givenname: C C surname: LaBranche fullname: LaBranche, C C organization: Hematology-Oncology Division, Hospital of the University of Pennsylvania, Philadelphia 19104, USA – sequence: 2 givenname: M M surname: Sauter fullname: Sauter, M M – sequence: 3 givenname: B S surname: Haggarty fullname: Haggarty, B S – sequence: 4 givenname: P J surname: Vance fullname: Vance, P J – sequence: 5 givenname: J surname: Romano fullname: Romano, J – sequence: 6 givenname: T K surname: Hart fullname: Hart, T K – sequence: 7 givenname: P J surname: Bugelski fullname: Bugelski, P J – sequence: 8 givenname: M surname: Marsh fullname: Marsh, M – sequence: 9 givenname: J A surname: Hoxie fullname: Hoxie, J A |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7636963$$D View this record in MEDLINE/PubMed |
| BookMark | eNpVkc9u1DAQxi1UVNrCIyDMhVuW2LHj-MChqvhTVIkLIG6W44x3XcV2sJOFfSjeEYddVfQ0kr_5Ps_M7xKdhRgAoVek3hBCu7efv99uWrmRG06JqDilYkOk5E_QBallV3FO2Bm6qGtKK950P56hy5zv65ow1rJzdC7appVtc4H-XOPswnYErL0LEWvjBmx2OmwBu4DnHWBzmOM06uydwUP0ujxH-0_JzjsdsPN-CXEA64yDYA5479KS8Zx0yB58XypgH0cwy7immgQ6Q8YQ9jDGCfB2PJg4pThDyYbfU4KcXSzflOxgwcxQZoJxzM_RU6vHDC9O9Qp9-_D-682n6u7Lx9ub67vKlEvMleC97hiVrDEdGaygpq-7nkgGouO1rRmxtBMtIbZljeTCMjnwFnhDhbA9pc0VenfMnZbew2AglGVGNSXndTqoqJ16rAS3U9u4V6STDSfF_-bkT_HnAnlW3uV1g3KJuGRF2o6RlonSKI6NJsWcE9iHP0itVtDqfu9UK5VUK2i1glYr6OJ8-f-ID74T2aK_Puo7t939cglUIfg4rfkLjza4AA |
| CitedBy_id | crossref_primary_10_1006_viro_2001_1177 crossref_primary_10_1128_JVI_74_13_5836_5844_2000 crossref_primary_10_1146_annurev_cellbio_20_010403_102350 crossref_primary_10_1016_j_vaccine_2017_03_062 crossref_primary_10_1074_jbc_273_2_950 crossref_primary_10_1074_jbc_M117_788919 crossref_primary_10_1128_JVI_78_24_13409_13419_2004 crossref_primary_10_1006_viro_1999_9637 crossref_primary_10_1016_S0962_8924_97_20038_3 crossref_primary_10_1002_pro_411 crossref_primary_10_1016_j_virol_2005_11_032 crossref_primary_10_1128_JVI_79_16_10210_10217_2005 crossref_primary_10_1099_vir_0_18589_0 crossref_primary_10_1016_j_jim_2011_05_010 crossref_primary_10_1073_pnas_0701443104 crossref_primary_10_1128_JVI_76_7_3338_3349_2002 crossref_primary_10_1006_viro_1998_9467 crossref_primary_10_1016_j_molimm_2004_06_028 crossref_primary_10_1371_journal_pone_0114753 crossref_primary_10_1074_jbc_M603609200 crossref_primary_10_1073_pnas_97_1_343 crossref_primary_10_1128_JVI_79_19_12455_12463_2005 crossref_primary_10_1034_j_1600_0854_2002_31004_x crossref_primary_10_1128_JVI_01049_07 crossref_primary_10_1089_aid_1998_14_373 crossref_primary_10_3390_v14010038 crossref_primary_10_1128_JVI_01515_09 crossref_primary_10_1128_JVI_73_2_1350_1361_1999 crossref_primary_10_1139_o06_176 crossref_primary_10_1128_JVI_77_12_6823_6835_2003 crossref_primary_10_1016_j_virol_2007_03_055 crossref_primary_10_1089_aid_2010_0281 crossref_primary_10_3390_v14081729 crossref_primary_10_1006_viro_2001_0869 crossref_primary_10_1089_aid_2010_0321 crossref_primary_10_1016_S0092_8674_00_81393_8 crossref_primary_10_1128_JVI_79_19_12231_12241_2005 crossref_primary_10_1128_JVI_01911_15 crossref_primary_10_2139_ssrn_3956653 crossref_primary_10_1091_mbc_e06_06_0535 crossref_primary_10_1016_j_bcmd_2005_06_006 crossref_primary_10_1111_j_1600_0684_1996_tb00013_x crossref_primary_10_1128_spectrum_02173_21 crossref_primary_10_1155_2014_157895 crossref_primary_10_1128_JVI_00415_17 crossref_primary_10_1128_JVI_01893_17 crossref_primary_10_1084_jem_20221654 crossref_primary_10_1128_JVI_01709_18 crossref_primary_10_1016_S0168_1702_98_00102_6 crossref_primary_10_1038_nature04817 crossref_primary_10_1089_0889222041524580 crossref_primary_10_1128_JVI_00149_17 crossref_primary_10_1128_JVI_73_11_9659_9663_1999 crossref_primary_10_1128_JVI_76_17_8875_8889_2002 crossref_primary_10_1128_JVI_77_2_1281_1291_2003 crossref_primary_10_1128_JVI_00958_20 crossref_primary_10_1128_JVI_75_20_9601_9612_2001 crossref_primary_10_1128_JVI_78_3_1069_1079_2004 crossref_primary_10_1016_j_virol_2004_06_041 crossref_primary_10_1097_00126334_200309010_00002 crossref_primary_10_1371_journal_pone_0033734 crossref_primary_10_1006_jmbi_1997_1277 crossref_primary_10_1016_S0042_6822_03_00297_6 crossref_primary_10_1128_CVI_00042_06 crossref_primary_10_1126_science_aaa9804 crossref_primary_10_1128_mbio_01825_21 crossref_primary_10_1128_JVI_73_12_10310_10319_1999 crossref_primary_10_1016_j_celrep_2015_08_055 crossref_primary_10_3390_v12020241 crossref_primary_10_1016_j_jviromet_2007_02_015 crossref_primary_10_1099_vir_0_80873_0 crossref_primary_10_1073_pnas_2123406119 crossref_primary_10_1074_jbc_M110315200 crossref_primary_10_1128_JVI_06463_11 crossref_primary_10_1128_JVI_00277_18 crossref_primary_10_1128_JVI_76_11_5315_5325_2002 crossref_primary_10_1128_JVI_76_6_2683_2691_2002 crossref_primary_10_1128_JVI_74_17_7922_7935_2000 crossref_primary_10_1128_JVI_73_6_5010_5017_1999 crossref_primary_10_1134_S0026893316030110 crossref_primary_10_1016_j_virol_2007_05_019 crossref_primary_10_1128_JVI_75_1_278_291_2001 crossref_primary_10_1128_MCB_20_1_249_260_2000 crossref_primary_10_1128_JVI_01254_13 crossref_primary_10_1128_JVI_80_9_4601_4609_2006 crossref_primary_10_1111_j_1348_0421_2008_00008_x crossref_primary_10_1128_JVI_76_22_11365_11378_2002 crossref_primary_10_1016_j_virol_2010_09_031 crossref_primary_10_1006_viro_1997_8839 crossref_primary_10_1371_journal_pone_0161596 crossref_primary_10_1128_JVI_01928_12 crossref_primary_10_1080_21505594_2022_2136433 crossref_primary_10_1016_j_virol_2006_06_023 crossref_primary_10_1128_JVI_76_1_327_337_2002 crossref_primary_10_1016_j_virol_2004_03_019 crossref_primary_10_1016_j_chom_2011_12_005 crossref_primary_10_1128_JVI_79_19_12311_12320_2005 crossref_primary_10_1016_j_virol_2004_03_016 crossref_primary_10_1371_journal_ppat_1003278 crossref_primary_10_1016_j_virol_2004_10_042 crossref_primary_10_1128_JVI_02053_12 crossref_primary_10_1128_JVI_72_12_10218_10221_1998 crossref_primary_10_1099_vir_0_009019_0 crossref_primary_10_1128_JVI_78_15_8301_8311_2004 crossref_primary_10_1186_1742_4690_8_37 crossref_primary_10_1016_j_virol_2017_10_013 crossref_primary_10_1128_JVI_73_5_4372_4384_1999 crossref_primary_10_1128_JVI_75_6_2982_2992_2001 crossref_primary_10_1128_JVI_01334_20 crossref_primary_10_1186_1742_4690_10_54 crossref_primary_10_1128_JVI_73_2_1293_1301_1999 crossref_primary_10_1006_viro_1997_8669 crossref_primary_10_1128_JVI_78_13_6775_6785_2004 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7U9 H94 5PM |
| DOI | 10.1128/JVI.69.9.5217-5227.1995 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Virology and AIDS Abstracts AIDS and Cancer Research Abstracts PubMed Central (Full Participant titles) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef AIDS and Cancer Research Abstracts Virology and AIDS Abstracts |
| DatabaseTitleList | CrossRef AIDS and Cancer Research Abstracts MEDLINE |
| Database_xml | – sequence: 1 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1098-5514 |
| EndPage | 5227 |
| ExternalDocumentID | 10_1128_jvi_69_9_5217_5227_1995 7636963 jvi_69_9_5217 |
| Genre | Research Support, U.S. Gov't, P.H.S Journal Article Comparative Study |
| GrantInformation_xml | – fundername: NIAID NIH HHS grantid: AI28208 – fundername: NIAID NIH HHS grantid: AI33854 |
| GroupedDBID | --- -~X .55 .GJ 0R~ 18M 29L 2WC 39C 3O- 4.4 41~ 53G 5GY 5RE 5VS 6TJ 85S AAYJJ ABPPZ ACGFO ACNCT ADBBV AENEX AFFNX AGVNZ AI. ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BTFSW C1A CGR CS3 CUY CVF D0S DIK E3Z EBS ECM EIF EJD F20 F5P FRP GX1 H13 HYE HZ~ IH2 KQ8 MVM N9A NPM O9- OHT OK1 P2P RHF RHI RNS RPM RSF TR2 UCJ UPT VH1 W2D W8F WH7 WOQ X7M Y6R YQT ZGI ZXP ~02 ~KM AAYXX CITATION 7U9 H94 5PM |
| ID | FETCH-LOGICAL-c522t-75ba842943c81df72cb08b194e7850f041f287611f643957f49d56e53277fb223 |
| IEDL.DBID | RPM |
| ISSN | 0022-538X |
| IngestDate | Tue Sep 17 21:00:43 EDT 2024 Fri Oct 25 04:50:50 EDT 2024 Thu Sep 12 16:49:38 EDT 2024 Sat Sep 28 07:35:28 EDT 2024 Wed May 18 15:27:50 EDT 2016 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 9 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c522t-75ba842943c81df72cb08b194e7850f041f287611f643957f49d56e53277fb223 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| OpenAccessLink | https://jvi.asm.org/content/jvi/69/9/5217.full.pdf |
| PMID | 7636963 |
| PQID | 16841647 |
| PQPubID | 23462 |
| PageCount | 11 |
| ParticipantIDs | proquest_miscellaneous_16841647 crossref_primary_10_1128_jvi_69_9_5217_5227_1995 pubmed_primary_7636963 highwire_asm_jvi_69_9_5217 pubmedcentral_primary_oai_pubmedcentral_nih_gov_189351 |
| PublicationCentury | 1900 |
| PublicationDate | 1995-09-01 |
| PublicationDateYYYYMMDD | 1995-09-01 |
| PublicationDate_xml | – month: 09 year: 1995 text: 1995-09-01 day: 01 |
| PublicationDecade | 1990 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Journal of Virology |
| PublicationTitleAlternate | J Virol |
| PublicationYear | 1995 |
| Publisher | American Society for Microbiology |
| Publisher_xml | – name: American Society for Microbiology |
| References | 7925266 - EMBO J. 1994 Sep 15;13(18):4204-11 6094591 - J Cell Biol. 1984 Dec;99(6):2011-23 1583738 - J Virol. 1992 Jun;66(6):3971-5 8030287 - Virology. 1994 Aug 15;203(1):8-19 1595905 - Anal Biochem. 1992 Jan;200(1):81-8 3646094 - Cell. 1987 May 8;49(3):307-19 1565636 - Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3443-7 1678438 - J Virol. 1991 Sep;65(9):4847-52 2839686 - J Virol. 1988 Aug;62(8):2557-68 7512161 - J Virol. 1994 May;68(5):3207-19 8107229 - J Virol. 1994 Mar;68(3):1689-96 1647069 - Virology. 1991 Jul;183(1):206-14 8195226 - J Biol Chem. 1994 Jun 3;269(22):15732-9 8497044 - J Virol. 1993 Jun;67(6):3077-86 2002555 - J Virol. 1991 Apr;65(4):2119-23 3041017 - J Virol. 1987 Oct;61(10):2981-8 2788444 - AIDS Res Hum Retroviruses. 1989 Aug;5(4):441-9 8396682 - J Virol. 1993 Oct;67(10):6289-94 8503172 - Virology. 1993 Jun;194(2):548-56 2243375 - J Virol. 1990 Dec;64(12):5701-7 7853546 - J Virol. 1995 Mar;69(3):1984-9 1322587 - Virology. 1992 Aug;189(2):534-46 2296088 - J Virol. 1990 Feb;64(2):922-6 1583717 - J Virol. 1992 Jun;66(6):3306-15 1986308 - Nature. 1991 Jan 10;349(6305):167-9 2002545 - J Virol. 1991 Apr;65(4):1843-54 2191297 - Proc Natl Acad Sci U S A. 1990 Jun;87(12):4650-4 8189506 - J Virol. 1994 Jun;68(6):3682-92 8254774 - J Virol. 1994 Jan;68(1):570-4 3038931 - J Cell Biol. 1987 Jul;105(1):69-75 8312049 - AIDS Res Hum Retroviruses. 1993 Nov;9(11):1057-66 3629244 - Science. 1987 Sep 11;237(4820):1351-5 2153143 - J Biol Chem. 1990 Jan 25;265(3):1777-82 1357190 - J Virol. 1992 Nov;66(11):6616-25 8057433 - J Virol. 1994 Sep;68(9):5509-22 6765172 - EMBO J. 1982;1(2):191-6 2547986 - J Virol. 1989 Sep;63(9):3569-78 1853560 - Virology. 1991 Aug;183(2):545-54 8416370 - J Virol. 1993 Jan;67(1):213-21 7933160 - J Virol. 1994 Nov;68(11):7665-7 7831790 - Virology. 1995 Jan 10;206(1):353-61 7734197 - AIDS Res Hum Retroviruses. 1995 Jan;11(1):55-64 8102410 - J Virol. 1993 Sep;67(9):5279-88 6352053 - Cell. 1983 Sep;34(2):513-24 1309251 - J Virol. 1992 Jan;66(1):349-58 2893293 - Nature. 1988 Feb 18;331(6157):619-22 8230430 - J Virol. 1993 Dec;67(12):7060-6 3011809 - J Cell Biol. 1986 Jun;102(6):2147-57 8226798 - J Biol Chem. 1993 Oct 25;268(30):22895-9 1985202 - J Virol. 1991 Jan;65(1):31-41 2557352 - J Cell Biol. 1989 Dec;109(6 Pt 2):3273-89 2778881 - J Virol. 1989 Oct;63(10):4395-403 8474176 - J Virol. 1993 May;67(5):2824-31 8289362 - J Virol. 1994 Feb;68(2):585-91 8207836 - J Virol. 1994 Jul;68(7):4620-7 3033661 - Proc Natl Acad Sci U S A. 1987 May;84(9):2756-60 7693966 - J Virol. 1993 Dec;67(12):7067-76 7957116 - EMBO J. 1994 Nov 15;13(22):5504-15 8212561 - Virology. 1993 Nov;197(1):255-64 8331736 - J Virol. 1993 Aug;67(8):4972-80 8411379 - J Virol. 1993 Nov;67(11):6762-7 8392617 - J Virol. 1993 Aug;67(8):4831-41 3144239 - Annu Rev Microbiol. 1988;42:489-516 1501283 - J Virol. 1992 Sep;66(9):5472-8 2398527 - J Virol. 1990 Oct;64(10):4678-83 8051216 - J Cell Biol. 1994 Aug;126(4):991-1004 8139010 - J Virol. 1994 Apr;68(4):2253-9 2109100 - J Virol. 1990 May;64(5):2149-56 7684467 - J Virol. 1993 Jun;67(6):3489-96 3014663 - Science. 1986 Aug 8;233(4764):655-9 8356808 - Virology. 1993 Sep;196(1):89-100 3028771 - EMBO J. 1986 Dec 1;5(12):3091-101 1309913 - J Virol. 1992 Feb;66(2):790-803 8474172 - J Virol. 1993 May;67(5):2747-55 2795718 - J Virol. 1989 Nov;63(11):4709-14 6325711 - J Mol Biol. 1984 Apr 5;174(2):297-317 2541505 - Science. 1989 May 12;244(4905):694-7 2023946 - Proc Natl Acad Sci U S A. 1991 May 1;88(9):3987-91 8280459 - Annu Rev Cell Biol. 1993;9:129-61 8035484 - J Virol. 1994 Aug;68(8):4857-61 |
| References_xml | |
| SSID | ssj0014464 |
| Score | 1.9058077 |
| Snippet | Article Usage Stats
Services
JVI
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley... We have described a virus termed CP-MAC, derived from the BK28 molecular clone of simian immunodeficiency virus, that was remarkable for its ability to infect... |
| SourceID | pubmedcentral proquest crossref pubmed highwire |
| SourceType | Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 5217 |
| SubjectTerms | Amino Acid Sequence Animals Antibodies, Monoclonal Base Sequence Cell Line Cell Membrane - metabolism Cloning, Molecular Cytoplasm - metabolism DNA Primers Flow Cytometry Gene Products, env - biosynthesis Gene Products, env - metabolism Genes, env Herpes Simplex Virus Protein Vmw65 - metabolism HIV - genetics Immunoblotting Kinetics Mice - immunology Microscopy, Immunoelectron Molecular Sequence Data Mutagenesis, Site-Directed Point Mutation Recombinant Proteins - biosynthesis Recombinant Proteins - metabolism Restriction Mapping Sequence Homology, Amino Acid simian immunodeficiency virus Simian Immunodeficiency Virus - genetics Simian Immunodeficiency Virus - metabolism Transcription Factors - metabolism |
| Title | A single amino acid change in the cytoplasmic domain of the simian immunodeficiency virus transmembrane molecule increases envelope glycoprotein expression on infected cells |
| URI | http://jvi.asm.org/content/69/9/5217.abstract https://www.ncbi.nlm.nih.gov/pubmed/7636963 https://search.proquest.com/docview/16841647 https://pubmed.ncbi.nlm.nih.gov/PMC189351 |
| Volume | 69 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELbYSpV6Qbwqtrx84JqsnTgPH6vSqiCBkHioNytxxmCUx6rZrdgf1f_YmTwqFnHiGie24xl7vrG_GTP2VpVOJIW0gbI6DRS6EIEWAgIVq8QKiKtxQ__yS_bpKn93Tmly1BwLM5D2benDtm7C1v8cuJXrxq5mntjq88cziUY2kasFWyA0nD306eQA3Rs1ZwjHyXw1cbpwGV59-P4-THWoQzRZGXpgUUaxeskRO8RJluo03rdNc77gf2HPvymUf9iki0fs4QQm-enY6cfsAbRP2OF4veTuKbs95bQTUAMvGt92vLC-4mOkL_ctR-jH7W7TrRFAN97yqmsKfNy5oaT3DWoO9xQ_0lVAiSYoSpPf-Ottzzdk4hpo0NdugTfjHbtUK4HQHnoO7UBGAv6j3tluyAaBdcPviXeLzWDdAxEMsE9Q1_0z9u3i_OvZZTBdzxBYHLtNkCVlkaM5U7FF0OuyyJYiL6VWkOWJcEJJh-5YKqUj1JNkTukqSSGJoyxzJcKSY3bQdi08Z1yAiHHtQLgQSSUrivbVlm5ryQsBzlVLJmbBmPWYhcMM3kuUm1833qTaaENSNSRVQ1JdspNZgAZHcf-1JXszi9TgdKK_xNHqtr2RKZ3DKnzjeBTwfXuTjixZuif5-3LK071fguo75Ose1fXkfz98wY7GOHoitr1kB5vrLbxii77avh5U_w4cAwog |
| link.rule.ids | 230,315,729,782,786,887,27935,27936,53803,53805 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwELYKFSoX6At1KS0-9JqsnTgPHxEFLRRQpdKKm5U4dusqjxXZReyP6n_sTB6oW3HimknsxPns-Sb5ZkzIJ5FbFmVce0LL2BMQQniSMeOJUESambDoP-jPviVXN-nnEyyTI8ZcmE60r3Pn12Xl1-5Xp62cV3o66sSmXy-POTjZiE83yHOYriwcY_Th3wEEOGKsEQ72m0HVBQvx9PzHmR9LX_rgtBKIwYIEs_WibbIF0yyWcbjuncaKwY-xz_9FlP94pdPdpz3PS7IzsFB61FtfkWemfk22-n0pV2_InyOKnxBKQ7PK1Q3NtCtonyJMXU2BM1K9WjRzYN6V07RoqgwON7aztK4CyFGHiSdNYbBCBaZ30jt3u2zpAn1jZSoI0mtDq35zXmwV2WtrWmrqTsVk6M9ypZuujAS0be4HwS50A213CjID92TKsn1Lvp-eXB_PvGFfB0_DkC-8JMqzFPygCDWwZZsEOmdpzqUwSRoxywS3EMfFnFukS1FihSyi2ERhkCQ2Bz6zRzbrpjbvCGWGhbDoAM8IuOAFpglLjdu8pBkz1hYTwsb3qeZ9-Q7VhT1Bqn7fORVLJRWCQSEYFIJhQvbH965gFNdPm5DDEQkK5iE-JYxWs2wVj_EHroAz9npcPPQ3QGtC4jXAPNixwPe6BXDSFfrucbH_1AsPyYvZ9eWFuji7-vKebPfJ-KiOOyCbi9ul-UA22mL5sZs9fwGuLh91 |
| linkToPdf | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LTtwwFLUKFRUb-kQd-sCLbpPYiZ3ESwSMoA-E1IfYWYljU1eJMyIzqPNR_GOv80Cdqiu2seMk9rHvuc651wh9YKUhvKAqYEqkAQMXIhCE6IAljCuik2rY0D_7ml1c5SenPk1OPsXC9KJ9VdrQ1U3o7M9eW7loVDTpxKLLL8cUjCyn0aIy0RZ6DFOW8MlPH_8fgJPDpjzhUH41KrtgMY4-_jgPUxGKEAxXBn5YnPmIPb6LdmCqpSJNNi3UlDX4fwz0XyHlX5Zp_vTh3_QM7Y1sFB8NNZ6jR9q9QDvD-ZTrl-juCPuthFrjorGuxYWyFR5ChbF1GLgjVutluwAG3liFq7Yp4HJr-pLONgA9bH0ASltpn6nCh3niW3uz6vDS28hGN-CsO42b4ZBe36pnsZ3usHa9mknj63qt2j6dBLStf4_CXXgMtN0ryTS8k67r7hX6Pj_9dnwWjOc7BAq6fRlkvCxysIcsUcCaTRarkuQlFUxnOSeGMGrAn0spNZ428cwwUfFU8yTOMlMCr9lH2651-jXCRJMEFh_gGzFltPLhwkL5417ygmhjqhki05jKxZDGQ_buT5zLX7dWpkIK6QEhPSCkB8QMHUxjL6EXN6vN0OGEBgnz0X8l9Fa76iRN_Y9cBjX2B2zcP2-E1wylG6C5L_eJvjdLACt9wu8BGwcPvfEQPbk8mcvP5xef3qDdISbfi-Teou3lzUq_Q1tdtXrfT6A_maYh9Q |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+single+amino+acid+change+in+the+cytoplasmic+domain+of+the+simian+immunodeficiency+virus+transmembrane+molecule+increases+envelope+glycoprotein+expression+on+infected+cells&rft.jtitle=Journal+of+virology&rft.au=LaBranche%2C+C+C&rft.au=Sauter%2C+M+M&rft.au=Haggarty%2C+B+S&rft.au=Vance%2C+P+J&rft.date=1995-09-01&rft.issn=0022-538X&rft.volume=69&rft.issue=9&rft.spage=5217&rft.epage=5227&rft_id=info:doi/10.1128%2FJVI.69.9.5217-5227.1995&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-538X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-538X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-538X&client=summon |